In this study, human meniscus tissue of normal origin in young adults and in patients with early and late stages of osteoarthritic degeneration were analyzed for their thermoanalytical properties with differential scanning calorimetry. Degenerative changes of knee menisci frequently result from trauma to the joint or are associated with joint diseases. Meniscus damage may play an important role in osteoarthritis pathophysiology. The purpose was to further characterize the altered metabolism in matrix composition during different stages of meniscus degeneration that promotes disease progression. The human knee joint menisectomy specimens were received after surgical removal. The calorimetric properties of samples were determined by DSC method, samples were heated from 0 to 80 C. The heating rate was 0.3 C min-1. Conventional Hastelloy batch vessels were used with 40 μL sample volume. Change in the enthalpy was observed in normal cartilage as 1632.48 J g-1 (SD = 50.55). In case of early degeneration a greater change at 1707.83 J g -1 (SD = 112.46), while in the severely degenerated samples at 1677.30 J g-1 (SD = 182.48) was measured. This method proved to be suitable for compositional thermoanalytical study of normal and degenerative human meniscus samples. All samples that were extracted for this study were obtained during live surgeries. With the rise of temperature an endothermic reaction was observed in all cases. The enthalpy change of the process initiated by the temperature change showed difference between the normal and pathological groups.
ASJC Scopus subject areas
- Condensed Matter Physics
- Physical and Theoretical Chemistry