Calcium entry is regulated by Zn2+ in relation to extracellular ionic environment in human airway epithelial cells

Dóra Hargitai, Ágnes Pataki, Gábor Raffai, Márta Füzi, Tamás Dankó, László Csernoch, Péter Várnai, Gyula Péter Szigeti, Ákos Zsembery

Research output: Article

9 Citations (Scopus)

Abstract

The extracellular pH, sodium and divalent cation concentrations influence the ATP-induced changes in cytosolic Ca2+ concentration ([Ca2+]i). This elevation of [Ca2+]i and activation of Ca2+-dependent Cl- channels represent a possible therapeutic approach in cystic fibrosis (CF). We investigated the changes of [Ca2+]i in different external ionic environment, and P2X purinergic receptors (P2XRs) expression in the control and CF airway epithelial cells. The parallel removal of Na+ and alkalinization of the extracellular solution increased the amplitude of sustained ATP-induced Ca2+ signals independent of wild-type or mutant CFTR expression. The ATP-induced Ca2+ entry was either inhibited or stimulated by Zn2+ depending on the extracellular Na+ concentration. In Na+-free environment, Zn2+ and other divalent cations elicited a biphasic Ca2+ signal. Immunohistochemical data suggest that, multiple subtypes of P2XRs are expressed in these airway epithelial cells. In conclusion, Ca2+ entry is finely regulated by external ionic environment. Therefore, we speculate that properly compiled aerosols could influence efficacy of zinc-based therapy in CF.

Original languageEnglish
Pages (from-to)67-75
Number of pages9
JournalRespiratory Physiology and Neurobiology
Volume170
Issue number1
DOIs
Publication statusPublished - jan. 31 2010

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Pulmonary and Respiratory Medicine

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