Caffeine and the myoplasmic calcium removal mechanisms in cut frog skeletal muscle fibres

L. Csernoch, L. Kovacs, B. Nilius, G. Szucs

Research output: Article

2 Citations (Scopus)


Antipyrylazo III myoplasmic calcium transients were recorded in cut skeletal muscle fibres of the frog (Rana esculenta), using the double vaseline-gap voltage-clamp system. Intracellular calcium removal mechanisms were analysed, using a slightly modified model taken from the literature. Parameter values reported here are generally consistent with those obtained by the original model. Caffeine (0.5 mmol .1-+) moderately enhanced the overall myoplasmic calcium removal. In particular, the rate constant of the non-saturable uptake increased by 51% on the average, but there was a considerable fiber-to-fiber variation. The kinetic features of the binding sites representing the saturable uptake did not change singificantly while the concentration of the available sites decreased by 36%. It is concluded that the caffeine-induced changes of the calcium removal components can be explained by supposing an increased resting myoplasmatic Ca2+ concentration in the presence of the drug.

Original languageEnglish
Pages (from-to)251-266
Number of pages16
JournalGeneral physiology and biophysics
Issue number3
Publication statusPublished - okt. 5 1990

ASJC Scopus subject areas

  • Biophysics
  • Physiology

Fingerprint Dive into the research topics of 'Caffeine and the myoplasmic calcium removal mechanisms in cut frog skeletal muscle fibres'. Together they form a unique fingerprint.

  • Cite this