c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription

Azadeh Arabi, Siqin Wu, Karin Ridderstråle, Holger Bierhoff, Chiounan Shiue, K. Fátyol, Sara Fahlén, Per Hydbring, Ola Söderberg, Ingrid Grummt, Lars Gunnar Larsson, Anthony P H Wright

Research output: Article

300 Citations (Scopus)

Abstract

The c-Myc oncoprotein regulates transcription of genes that are associated with cell growth, proliferation and apoptosis. c-Myc levels are modulated by ubiquitin/proteasome-mediated degradation. Proteasome inhibition leads to c-Myc accumulation within nucleoli, indicating that c-Myc might have a nucleolar function. Here we show that the proteins c-Myc and Max interact in nucleoli and are associated with ribosomal DNA. This association is increased upon activation of quiescent cells and is followed by recruitment of the Myc cofactor TRRAP, enhanced histone acetylation, recruitment of RNA polymerase I (Pol I), and activation of rDNA transcription. Using small interfering RNAs (siRNAs) against c-Myc and an inhibitor of Myc-Max interactions, we demonstrate that c-Myc is required for activating rDNA transcription in response to mitogenic signals. Furthermore, using the ligand-activated MycER (ER, oestrogen receptor) system, we show that c-Myc can activate Pol I transcription in the absence of Pol II transcription. These results suggest that c-Myc coordinates the activity of all three nuclear RNA polymerases, and thereby plays a key role in regulating ribosome biogenesis and cell growth.

Original languageEnglish
Pages (from-to)303-310
Number of pages8
JournalNature Cell Biology
Volume7
Issue number3
DOIs
Publication statusPublished - márc. 2005

Fingerprint

RNA Polymerase I
Ribosomal DNA
Proteasome Endopeptidase Complex
Proto-Oncogene Proteins c-myc
Nuclear RNA
Oncogene Proteins
DNA-Directed RNA Polymerases
Acetylation
Growth
Ubiquitin
Ribosomes
Estrogen Receptors
Histones
Small Interfering RNA
Transcriptional Activation
Cell Proliferation
Apoptosis
Ligands
Genes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Arabi, A., Wu, S., Ridderstråle, K., Bierhoff, H., Shiue, C., Fátyol, K., ... Wright, A. P. H. (2005). c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription. Nature Cell Biology, 7(3), 303-310. https://doi.org/10.1038/ncb1225

c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription. / Arabi, Azadeh; Wu, Siqin; Ridderstråle, Karin; Bierhoff, Holger; Shiue, Chiounan; Fátyol, K.; Fahlén, Sara; Hydbring, Per; Söderberg, Ola; Grummt, Ingrid; Larsson, Lars Gunnar; Wright, Anthony P H.

In: Nature Cell Biology, Vol. 7, No. 3, 03.2005, p. 303-310.

Research output: Article

Arabi, A, Wu, S, Ridderstråle, K, Bierhoff, H, Shiue, C, Fátyol, K, Fahlén, S, Hydbring, P, Söderberg, O, Grummt, I, Larsson, LG & Wright, APH 2005, 'c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription', Nature Cell Biology, vol. 7, no. 3, pp. 303-310. https://doi.org/10.1038/ncb1225
Arabi, Azadeh ; Wu, Siqin ; Ridderstråle, Karin ; Bierhoff, Holger ; Shiue, Chiounan ; Fátyol, K. ; Fahlén, Sara ; Hydbring, Per ; Söderberg, Ola ; Grummt, Ingrid ; Larsson, Lars Gunnar ; Wright, Anthony P H. / c-Myc associates with ribosomal DNA and activates RNA polymerase I transcription. In: Nature Cell Biology. 2005 ; Vol. 7, No. 3. pp. 303-310.
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