C-myc amplification and cluster analysis in human gastric carcinoma

L. Kozma, I. Kiss, J. Hajdú, Z. Szentkereszty, S. Szakáll, I. Ember

Research output: Article

27 Citations (Scopus)

Abstract

The tumour samples of 23 patients (9 male, 14 female, aged 28-85) were randomly selected for the study. DNA was isolated from paraffin embedded tissue for quantitative dot-blot hybridization, in order to determine the amplification values for the c-myc and K-fas oncogenes. The clinical and histological parameters studied were as follows: grade, TNM staging system, Lauren's histological type, localization and the severity of the disease. Amplified c-myc was found in 6 cases. Amplification was concomitant with c-myc overexpression detected with immunohistochemical staining. The amplification - 9.1-fold on the average (ranging from 2.12 to 18.2) was significantly associated with the presence of distant metastasis (corr. coeff.: 0.5623, p<0.01), but with none of the other parameters. No case with K-ras amplification was recorded. The result of the multivariate cluster analysis proved that age was the decisive factor in the segregation process. This age-related distribution (69 vs. 40, p<0.001), however, did not coincide with either the incidence of distant metastasis or c-myc amplification.

Original languageEnglish
Pages (from-to)707-710
Number of pages4
JournalAnticancer research
Volume21
Issue number1 B
Publication statusPublished - ápr. 17 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Kozma, L., Kiss, I., Hajdú, J., Szentkereszty, Z., Szakáll, S., & Ember, I. (2001). C-myc amplification and cluster analysis in human gastric carcinoma. Anticancer research, 21(1 B), 707-710.