Brain protein expression changes in WAG/Rij rats, a genetic rat model of absence epilepsy after peripheral lipopolysaccharide treatment

B. Györffy, Z. Kovács, Péter Gulyássy, Attila Simor, Katalin Völgyi, Gergely Orbán, Péter Baracskay, Zoltán Szabó, T. Janáky, A. Dobolyi, G. Juhász, A. Czurkó, K. Kékesi

Research output: Article

14 Citations (Scopus)

Abstract

Peripheral injection of bacterial lipopolysaccharide (LPS) facilitates 8-10. Hz spike-wave discharges (SWD) characterizing absence epilepsy in WAG/Rij rats. It is unknown however, whether peripherally administered LPS is able to alter the generator areas of epileptic activity at the molecular level. We injected 1. mg/kg dose of LPS intraperitoneally into WAG/Rij rats, recorded the body temperature and EEG, and examined the protein expression changes of the proteome 12. h after injection in the fronto-parietal cortex and thalamus. We used fluorescent two-dimensional differential gel electrophoresis to investigate the expression profile. We found 16 differentially expressed proteins in the fronto-parietal cortex and 35 proteins in the thalamus. It is known that SWD genesis correlates with the transitional state of sleep-wake cycle thus we performed meta-analysis of the altered proteins in relation to inflammation, epilepsy as well as sleep. The analysis revealed that all categories are highly represented by the altered proteins and these protein-sets have considerable overlap. Protein network modeling suggested that the alterations in the proteome were largely induced by the immune response, which invokes the NFkB signaling pathway. The proteomics and computational analysis verified the known functional interplay between inflammation, epilepsy and sleep and highlighted proteins that are involved in their common synaptic mechanisms. Our physiological findings support the phenomenon that high dose of peripheral LPS injection increases SWD-number, modifies its duration as well as the sleep-wake stages and decreases body temperature.

Original languageEnglish
Pages (from-to)86-95
Number of pages10
JournalBrain, Behavior, and Immunity
Volume35
DOIs
Publication statusPublished - jan. 2014

Fingerprint

Absence Epilepsy
Genetic Models
Lipopolysaccharides
Brain
Proteins
Sleep
Parietal Lobe
Proteome
Therapeutics
Body Temperature
Thalamus
Injections
Epilepsy
Inflammation
Sleep Stages
Electrophoresis, Gel, Two-Dimensional
Proteomics
Meta-Analysis
Electroencephalography

ASJC Scopus subject areas

  • Immunology
  • Behavioral Neuroscience
  • Endocrine and Autonomic Systems
  • Medicine(all)

Cite this

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title = "Brain protein expression changes in WAG/Rij rats, a genetic rat model of absence epilepsy after peripheral lipopolysaccharide treatment",
abstract = "Peripheral injection of bacterial lipopolysaccharide (LPS) facilitates 8-10. Hz spike-wave discharges (SWD) characterizing absence epilepsy in WAG/Rij rats. It is unknown however, whether peripherally administered LPS is able to alter the generator areas of epileptic activity at the molecular level. We injected 1. mg/kg dose of LPS intraperitoneally into WAG/Rij rats, recorded the body temperature and EEG, and examined the protein expression changes of the proteome 12. h after injection in the fronto-parietal cortex and thalamus. We used fluorescent two-dimensional differential gel electrophoresis to investigate the expression profile. We found 16 differentially expressed proteins in the fronto-parietal cortex and 35 proteins in the thalamus. It is known that SWD genesis correlates with the transitional state of sleep-wake cycle thus we performed meta-analysis of the altered proteins in relation to inflammation, epilepsy as well as sleep. The analysis revealed that all categories are highly represented by the altered proteins and these protein-sets have considerable overlap. Protein network modeling suggested that the alterations in the proteome were largely induced by the immune response, which invokes the NFkB signaling pathway. The proteomics and computational analysis verified the known functional interplay between inflammation, epilepsy and sleep and highlighted proteins that are involved in their common synaptic mechanisms. Our physiological findings support the phenomenon that high dose of peripheral LPS injection increases SWD-number, modifies its duration as well as the sleep-wake stages and decreases body temperature.",
keywords = "Brain proteomics, Epilepsy, Inflammation, LPS, NFkB, Sleep-wake cycle",
author = "B. Gy{\"o}rffy and Z. Kov{\'a}cs and P{\'e}ter Guly{\'a}ssy and Attila Simor and Katalin V{\"o}lgyi and Gergely Orb{\'a}n and P{\'e}ter Baracskay and Zolt{\'a}n Szab{\'o} and T. Jan{\'a}ky and A. Dobolyi and G. Juh{\'a}sz and A. Czurk{\'o} and K. K{\'e}kesi",
year = "2014",
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T1 - Brain protein expression changes in WAG/Rij rats, a genetic rat model of absence epilepsy after peripheral lipopolysaccharide treatment

AU - Györffy, B.

AU - Kovács, Z.

AU - Gulyássy, Péter

AU - Simor, Attila

AU - Völgyi, Katalin

AU - Orbán, Gergely

AU - Baracskay, Péter

AU - Szabó, Zoltán

AU - Janáky, T.

AU - Dobolyi, A.

AU - Juhász, G.

AU - Czurkó, A.

AU - Kékesi, K.

PY - 2014/1

Y1 - 2014/1

N2 - Peripheral injection of bacterial lipopolysaccharide (LPS) facilitates 8-10. Hz spike-wave discharges (SWD) characterizing absence epilepsy in WAG/Rij rats. It is unknown however, whether peripherally administered LPS is able to alter the generator areas of epileptic activity at the molecular level. We injected 1. mg/kg dose of LPS intraperitoneally into WAG/Rij rats, recorded the body temperature and EEG, and examined the protein expression changes of the proteome 12. h after injection in the fronto-parietal cortex and thalamus. We used fluorescent two-dimensional differential gel electrophoresis to investigate the expression profile. We found 16 differentially expressed proteins in the fronto-parietal cortex and 35 proteins in the thalamus. It is known that SWD genesis correlates with the transitional state of sleep-wake cycle thus we performed meta-analysis of the altered proteins in relation to inflammation, epilepsy as well as sleep. The analysis revealed that all categories are highly represented by the altered proteins and these protein-sets have considerable overlap. Protein network modeling suggested that the alterations in the proteome were largely induced by the immune response, which invokes the NFkB signaling pathway. The proteomics and computational analysis verified the known functional interplay between inflammation, epilepsy and sleep and highlighted proteins that are involved in their common synaptic mechanisms. Our physiological findings support the phenomenon that high dose of peripheral LPS injection increases SWD-number, modifies its duration as well as the sleep-wake stages and decreases body temperature.

AB - Peripheral injection of bacterial lipopolysaccharide (LPS) facilitates 8-10. Hz spike-wave discharges (SWD) characterizing absence epilepsy in WAG/Rij rats. It is unknown however, whether peripherally administered LPS is able to alter the generator areas of epileptic activity at the molecular level. We injected 1. mg/kg dose of LPS intraperitoneally into WAG/Rij rats, recorded the body temperature and EEG, and examined the protein expression changes of the proteome 12. h after injection in the fronto-parietal cortex and thalamus. We used fluorescent two-dimensional differential gel electrophoresis to investigate the expression profile. We found 16 differentially expressed proteins in the fronto-parietal cortex and 35 proteins in the thalamus. It is known that SWD genesis correlates with the transitional state of sleep-wake cycle thus we performed meta-analysis of the altered proteins in relation to inflammation, epilepsy as well as sleep. The analysis revealed that all categories are highly represented by the altered proteins and these protein-sets have considerable overlap. Protein network modeling suggested that the alterations in the proteome were largely induced by the immune response, which invokes the NFkB signaling pathway. The proteomics and computational analysis verified the known functional interplay between inflammation, epilepsy and sleep and highlighted proteins that are involved in their common synaptic mechanisms. Our physiological findings support the phenomenon that high dose of peripheral LPS injection increases SWD-number, modifies its duration as well as the sleep-wake stages and decreases body temperature.

KW - Brain proteomics

KW - Epilepsy

KW - Inflammation

KW - LPS

KW - NFkB

KW - Sleep-wake cycle

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JO - Brain, Behavior, and Immunity

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