Biological effect of He‐Ne laser: Investigations on functional and micromorphological alterations of cell membranes, in vitro

Tamara Kubasova, Zoltán Somosy, Péter Unk, András Kókai, Lajos Kovács

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A 1‐J/cm2 dose of single laser‐irradiation applied on primary human embryo fibroblast culture was not followed either by functional or micromorphological alterations of cell surfaces. This dose, however, applied four times with 24‐hour intervals changed the functional conditions as well as surface charges of cell membranes. As detection methods, radioactive glucosamine uptake, lectin, and cationized ferritin‐binding techniques were applied. At the same time, the scanning and transmission electron microscopy of laser‐irradiated cells did not reveal any micromorphological or ultrastructural alterations. The effect of a 5‐J/cm2dose did not differ from that of 1 J/cm2. It is suggested that laser radiation‐induced circumstances on cell surfaces can contribute to the strength of cell‐to‐cell contacts, i.e., to the stimulation of epithelization experienced in the clinical gynecol‐ ogic practice.

Original languageEnglish
Pages (from-to)381-388
Number of pages8
JournalLasers in Surgery and Medicine
Issue number4
Publication statusPublished - 1984


ASJC Scopus subject areas

  • Surgery
  • Dermatology

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