Bioactivity-guided isolation of cytotoxic sesquiterpenes and flavonoids from anthemis ruthenica

Research output: Article

14 Citations (Scopus)


A new eudesmanolide sesquiterpene, sivasinolide 6-O-angelate (1), was isolated from the aerial parts of Anthemis ruthenica together with the known compounds chrysanin (2), tanacin (3), 3-hydroxycostunolide (4), centauridin (5), and centaureidin (6). The compounds were obtained by means of bioactivity-guided fractionation from the CHCl3 extract of the herb, which displayed high cytotoxic activity. The structures were determined by UV, HRESIMS, and high-field 1D and 2DNMR spectral analyses, affording complete 1H-and 13CNMR assignments for all compounds. The cytotoxic activities of the isolated sesquiterpenes and flavonoids were assessed against cervical adenocarcinoma HeLa, breast adenocarcinoma MCF7, and skin epidermoid carcinoma A431 cells using the MTT assay. It was found that, apart from centaureidin (6), which is extremely active (IC50 0.082, 0.13, and 0.35μ M on the HeLa, MCF7, and A431 cell lines, respectively), all these compounds exert high or moderate tumor cell-growth inhibitory activity (IC 50 3.4258.15μM).

Original languageEnglish
Pages (from-to)94-96
Number of pages3
JournalPlanta Medica
Issue number1
Publication statusPublished - jan. 25 2010

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Bioactivity-guided isolation of cytotoxic sesquiterpenes and flavonoids from anthemis ruthenica'. Together they form a unique fingerprint.

  • Cite this