Binding constant of VIVO to transferrin

T. Kiss, T. Jakusch, Saâd Bouhsina, Hiromu Sakurai, E. Enyedy

Research output: Article

35 Citations (Scopus)

Abstract

The interactions of bis-VIVO-1,2-dimethyl-3-hydroxy-4(1H)- pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed, and the reaction was used to determine the binding constant of V IVO to apoTF. A value of log K = 14.3±0.6 was obtained for the binding of the first VIVO to apoTF. In order to confirm the validity of the binding constant, UV spectroscopy was used to monitor the displacement reaction between VIVO-apoTF and nitrilotriacetic acid (NTA). The difference between the binding constants obtained by the two different methods (ca. one log unit) was explained in terms of the possible ternary complex formation between VIVO-apoTF and the displacing ligand. The binding constants obtained exhibit a reasonably good agreement with the linear free energy relationship (LFER) estimation.

Original languageEnglish
Pages (from-to)3607-3613
Number of pages7
JournalEuropean Journal of Inorganic Chemistry
Issue number18
DOIs
Publication statusPublished - szept. 18 2006

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Transferrin
Nitrilotriacetic Acid
Ligands
Liquid nitrogen
Ultraviolet spectroscopy
Free energy
Paramagnetic resonance
apotransferrin
Spectroscopy
Temperature

ASJC Scopus subject areas

  • Inorganic Chemistry

Cite this

Binding constant of VIVO to transferrin. / Kiss, T.; Jakusch, T.; Bouhsina, Saâd; Sakurai, Hiromu; Enyedy, E.

In: European Journal of Inorganic Chemistry, No. 18, 18.09.2006, p. 3607-3613.

Research output: Article

Kiss, T. ; Jakusch, T. ; Bouhsina, Saâd ; Sakurai, Hiromu ; Enyedy, E. / Binding constant of VIVO to transferrin. In: European Journal of Inorganic Chemistry. 2006 ; No. 18. pp. 3607-3613.
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AU - Bouhsina, Saâd

AU - Sakurai, Hiromu

AU - Enyedy, E.

PY - 2006/9/18

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