Avian coronavirus infection induces mannose-binding lectin production in dendritic cell precursors of chicken lymphoid organs

Attila Farsang, Ildikó Bódi, Orsolya Fölker, Krisztina Minkó, Zsófia Benyeda, A. Bálint, Imre Oláh

Research output: Article

Abstract

The aim of this immunocytochemical study was to compare mannose-binding lectin (MBL) production induced by avian coronavirus in the spleen and caecal tonsil (CT). One-day-old specific-pathogen-free (SPF) chickens were experimentally infected with six QX field isolates and the H120 vaccine strain. In the negative control birds, the spleen was MBL negative, while the CT showed scattered MBL-positive cells in close proximity and within the surface epithelium and germinal centre (GC)-like cell clusters. MBL was detectable in the ellipsoid-associated cells (EACs) and cell clusters in the periarterial lymphoid sheath (PALS) by 7 days post infection (dpi). In both organs, the MBL-positive cells occupy antigen-exposed areas, indicating that GC formation depends on resident precursors of dendritic cells. The majority of MBL-positive EACs express the CD83 antigen, providing evidence that coronavirus infection facilitated the maturation of dendritic cell precursors. Surprisingly, co-localisation of MBL and CD83 was not detectable in the CT. In the spleen (associated with circulation), the EACs producing MBL and expressing CD83 are a common precursor of both follicular (FDC) and interdigitating dendritic cells (IDC). In the CT (gut-associated lymphoid tissue, GALT) the precursors of FDC and IDC are MBL-producing cells and CD83-positive cells, respectively. In the CT the two separate precursors of lymphoid dendritic cells provide some 'autonomy' for the GALT.

Original languageEnglish
Pages (from-to)183-196
Number of pages14
JournalActa veterinaria Hungarica
Volume67
Issue number2
DOIs
Publication statusPublished - jún. 1 2019

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Avian coronavirus
Coronavirus Infections
Mannose-Binding Lectin
dendritic cells
mannose
lectins
Dendritic Cells
Chickens
chickens
tonsils
Palatine Tonsil
infection
cells
spleen
Germinal Center
Spleen
Lymphoid Tissue
digestive system
Follicular Dendritic Cells
antigens

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Avian coronavirus infection induces mannose-binding lectin production in dendritic cell precursors of chicken lymphoid organs. / Farsang, Attila; Bódi, Ildikó; Fölker, Orsolya; Minkó, Krisztina; Benyeda, Zsófia; Bálint, A.; Oláh, Imre.

In: Acta veterinaria Hungarica, Vol. 67, No. 2, 01.06.2019, p. 183-196.

Research output: Article

Farsang, Attila ; Bódi, Ildikó ; Fölker, Orsolya ; Minkó, Krisztina ; Benyeda, Zsófia ; Bálint, A. ; Oláh, Imre. / Avian coronavirus infection induces mannose-binding lectin production in dendritic cell precursors of chicken lymphoid organs. In: Acta veterinaria Hungarica. 2019 ; Vol. 67, No. 2. pp. 183-196.
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