Automated four-color analysis of leukocytes by scanning fluorescence microscopy using quantum dots

József Bocsi, Dominik Lenz, Anja Mittag, Viktor Sebestyén Varga, Béla Molnar, Zsolt Tulassay, Ulrich Sack, Attila Tarnok

Research output: Article

18 Citations (Scopus)

Abstract

Background: Scanning fluorescence microscope (SFM) is a new technique for automated motorized microscopes to measure multiple fluorochrome labeled cells (Bocsi et al., Cytometry A 2004, 61:1-8). Aims: We developed a four-color staining protocol (DNA, CD3, CD4, and CD8) for the lymphocyte phenotyping by SFM. Methods: Organic (Alexa488, FITC, PE-Alexa610, CyChrom, APC) and inorganic (quantum dot (QD) 605 or 655) fluorochromes were used and compared in different combinations. Measurements were performed in suspension by flow cytometer (FCM) and on slide by SFM. Results: Both QDs were detectable by the appropriate Axioplan-2 and FCM filters and the AxioCam BW-camera. CD4/CD8 ratios were highly correlated (P = 0.01) between the SFM and FCM. Conclusion: Automated SFM is an applicable tool for CD4/CD8 ratio determination in peripheral blood samples with QDs.

Original languageEnglish
Pages (from-to)131-134
Number of pages4
JournalCytometry Part A
Volume69
Issue number3
DOIs
Publication statusPublished - márc. 1 2006

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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