Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity

Zsuzsanna Mérai, Z. Kerényi, Attila Molnár, E. Barta, Anna Válóczi, György Bisztray, Z. Havelda, J. Burgyán, D. Silhavy

Research output: Article

93 Citations (Scopus)

Abstract

RNA silencing is a conserved eukaryotic gene regulatory system in which sequence specificity is determined by small RNAs. Plant RNA silencing also acts as an antiviral mechanism; therefore, viral infection requires expression of a silencing suppressor. The mechanism and the evolution of silencing suppression are still poorly understood. Tombusvirus open reading frame (ORF) 5-encoded P19 is a size-selective double-stranded RNA (dsRNA) binding protein that suppresses silencing by sequestering double-stranded small interfering RNAs (siRNAs), the specificity determinant of the antiviral silencing system. To better understand the evolution of silencing suppression, we characterized the suppressor of the type member of Aureusviruses, the closest relatives of the genus Tombusvirus. We show that the Pothos latent virus (PoLV) ORF 5-encoded P14 is an efficient suppressor of both virus- and transgene-induced silencing. Findings that in vitro P14 binds dsRNAs and double-stranded siRNAs without obvious size selection suggest that P14, unlike P19, can suppress silencing by sequestering both long dsRNA and double-stranded siRNA components of the silencing machinery. Indeed, P14 prevents the accumulation of hairpin transcript-derived siRNAs, indicating that P14 inhibits inverted repeat-induced silencing by binding the long dsRNA precursors of siRNAs. However, viral siRNAs accumulate to high levels in PoLV-infected plants; therefore, P14 might inhibit virus-induced silencing by sequestering double-stranded siRNAs. Finally, sequence analyses suggest that P14 and P19 suppressors diverged from an ancient dsRNA binding suppressor that evolved as a nested protein within the common ancestor of aureusvirus- tombusvirus movement proteins.

Original languageEnglish
Pages (from-to)7217-7226
Number of pages10
JournalJournal of Virology
Volume79
Issue number11
DOIs
Publication statusPublished - jún. 2005

Fingerprint

Aureusvirus
Tombusviridae
Double-Stranded RNA
double-stranded RNA
small interfering RNA
RNA Interference
RNA interference
Small Interfering RNA
Tombusvirus
Pothos latent virus
Viruses
Open Reading Frames
Antiviral Agents
open reading frames
Plant RNA
Plant Viruses
viruses
RNA-binding proteins
RNA-Binding Proteins
RNA Precursors

ASJC Scopus subject areas

  • Immunology

Cite this

Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity. / Mérai, Zsuzsanna; Kerényi, Z.; Molnár, Attila; Barta, E.; Válóczi, Anna; Bisztray, György; Havelda, Z.; Burgyán, J.; Silhavy, D.

In: Journal of Virology, Vol. 79, No. 11, 06.2005, p. 7217-7226.

Research output: Article

@article{657be97213a64aedaecab36507ced5ad,
title = "Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity",
abstract = "RNA silencing is a conserved eukaryotic gene regulatory system in which sequence specificity is determined by small RNAs. Plant RNA silencing also acts as an antiviral mechanism; therefore, viral infection requires expression of a silencing suppressor. The mechanism and the evolution of silencing suppression are still poorly understood. Tombusvirus open reading frame (ORF) 5-encoded P19 is a size-selective double-stranded RNA (dsRNA) binding protein that suppresses silencing by sequestering double-stranded small interfering RNAs (siRNAs), the specificity determinant of the antiviral silencing system. To better understand the evolution of silencing suppression, we characterized the suppressor of the type member of Aureusviruses, the closest relatives of the genus Tombusvirus. We show that the Pothos latent virus (PoLV) ORF 5-encoded P14 is an efficient suppressor of both virus- and transgene-induced silencing. Findings that in vitro P14 binds dsRNAs and double-stranded siRNAs without obvious size selection suggest that P14, unlike P19, can suppress silencing by sequestering both long dsRNA and double-stranded siRNA components of the silencing machinery. Indeed, P14 prevents the accumulation of hairpin transcript-derived siRNAs, indicating that P14 inhibits inverted repeat-induced silencing by binding the long dsRNA precursors of siRNAs. However, viral siRNAs accumulate to high levels in PoLV-infected plants; therefore, P14 might inhibit virus-induced silencing by sequestering double-stranded siRNAs. Finally, sequence analyses suggest that P14 and P19 suppressors diverged from an ancient dsRNA binding suppressor that evolved as a nested protein within the common ancestor of aureusvirus- tombusvirus movement proteins.",
author = "Zsuzsanna M{\'e}rai and Z. Ker{\'e}nyi and Attila Moln{\'a}r and E. Barta and Anna V{\'a}l{\'o}czi and Gy{\"o}rgy Bisztray and Z. Havelda and J. Burgy{\'a}n and D. Silhavy",
year = "2005",
month = "6",
doi = "10.1128/JVI.79.11.7217-7226.2005",
language = "English",
volume = "79",
pages = "7217--7226",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Aureusvirus P14 is an efficient RNA silencing suppressor that binds double-stranded RNAs without size specificity

AU - Mérai, Zsuzsanna

AU - Kerényi, Z.

AU - Molnár, Attila

AU - Barta, E.

AU - Válóczi, Anna

AU - Bisztray, György

AU - Havelda, Z.

AU - Burgyán, J.

AU - Silhavy, D.

PY - 2005/6

Y1 - 2005/6

N2 - RNA silencing is a conserved eukaryotic gene regulatory system in which sequence specificity is determined by small RNAs. Plant RNA silencing also acts as an antiviral mechanism; therefore, viral infection requires expression of a silencing suppressor. The mechanism and the evolution of silencing suppression are still poorly understood. Tombusvirus open reading frame (ORF) 5-encoded P19 is a size-selective double-stranded RNA (dsRNA) binding protein that suppresses silencing by sequestering double-stranded small interfering RNAs (siRNAs), the specificity determinant of the antiviral silencing system. To better understand the evolution of silencing suppression, we characterized the suppressor of the type member of Aureusviruses, the closest relatives of the genus Tombusvirus. We show that the Pothos latent virus (PoLV) ORF 5-encoded P14 is an efficient suppressor of both virus- and transgene-induced silencing. Findings that in vitro P14 binds dsRNAs and double-stranded siRNAs without obvious size selection suggest that P14, unlike P19, can suppress silencing by sequestering both long dsRNA and double-stranded siRNA components of the silencing machinery. Indeed, P14 prevents the accumulation of hairpin transcript-derived siRNAs, indicating that P14 inhibits inverted repeat-induced silencing by binding the long dsRNA precursors of siRNAs. However, viral siRNAs accumulate to high levels in PoLV-infected plants; therefore, P14 might inhibit virus-induced silencing by sequestering double-stranded siRNAs. Finally, sequence analyses suggest that P14 and P19 suppressors diverged from an ancient dsRNA binding suppressor that evolved as a nested protein within the common ancestor of aureusvirus- tombusvirus movement proteins.

AB - RNA silencing is a conserved eukaryotic gene regulatory system in which sequence specificity is determined by small RNAs. Plant RNA silencing also acts as an antiviral mechanism; therefore, viral infection requires expression of a silencing suppressor. The mechanism and the evolution of silencing suppression are still poorly understood. Tombusvirus open reading frame (ORF) 5-encoded P19 is a size-selective double-stranded RNA (dsRNA) binding protein that suppresses silencing by sequestering double-stranded small interfering RNAs (siRNAs), the specificity determinant of the antiviral silencing system. To better understand the evolution of silencing suppression, we characterized the suppressor of the type member of Aureusviruses, the closest relatives of the genus Tombusvirus. We show that the Pothos latent virus (PoLV) ORF 5-encoded P14 is an efficient suppressor of both virus- and transgene-induced silencing. Findings that in vitro P14 binds dsRNAs and double-stranded siRNAs without obvious size selection suggest that P14, unlike P19, can suppress silencing by sequestering both long dsRNA and double-stranded siRNA components of the silencing machinery. Indeed, P14 prevents the accumulation of hairpin transcript-derived siRNAs, indicating that P14 inhibits inverted repeat-induced silencing by binding the long dsRNA precursors of siRNAs. However, viral siRNAs accumulate to high levels in PoLV-infected plants; therefore, P14 might inhibit virus-induced silencing by sequestering double-stranded siRNAs. Finally, sequence analyses suggest that P14 and P19 suppressors diverged from an ancient dsRNA binding suppressor that evolved as a nested protein within the common ancestor of aureusvirus- tombusvirus movement proteins.

UR - http://www.scopus.com/inward/record.url?scp=18744381842&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18744381842&partnerID=8YFLogxK

U2 - 10.1128/JVI.79.11.7217-7226.2005

DO - 10.1128/JVI.79.11.7217-7226.2005

M3 - Article

C2 - 15890960

AN - SCOPUS:18744381842

VL - 79

SP - 7217

EP - 7226

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 11

ER -