ATP and Integrity of Human Red Blood Cells

Sándor Nagy, Mária Paál, Tamás Kõszegi, Andrea Ludány, Miklós Kellermayer

Research output: Article

8 Citations (Scopus)

Abstract

In spite of the well known significance of ATP in the energy dependent life processes, the role of ATP in maintaining cellular integrity is poorly understood. A possible model for studying ATP dependent life processes is to monitor the kinetics of changes seen intra/extracellularly during ATP depletion. In our model system anticoagulated human whole blood was incubated at different temperatures to reduce intracellular ATP without addition of any chemicals. The red blood cells in their own plasma were incubated for several days at 4°C or at 37°CATP, glucose, K+, Na+, hemoglobin, water content, mean corpuscular volume (MCV), pH and Ca2+ were analyzed in time-sequences. All the examined parameters remained practically unchanged at 4°C, while at 37°C total ATP and glucose decreased parallel and after a transient increase of MCV, the water content of red blood cells decreased. As the actual ATP fell below 10% of the initial ATP content (at 48 h), the release of potassium sharply increased. Release of hemoglobin started only after 96 hours of incubation. Maximums of changes of the examined parameters were found at different time intervals. The maximal speed of concentration changes for glucose was found at 12-24 hours of incubation and at 24-36 hours for ATP, at 48-60 hours for K+-Na+ and after 96 hours for hemoglobin.

Original languageEnglish
Pages (from-to)141-148
Number of pages8
JournalPhysiological chemistry and physics and medical NMR
Volume30
Issue number2
Publication statusPublished - dec. 1 1998

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Physiology
  • Spectroscopy

Cite this

Nagy, S., Paál, M., Kõszegi, T., Ludány, A., & Kellermayer, M. (1998). ATP and Integrity of Human Red Blood Cells. Physiological chemistry and physics and medical NMR, 30(2), 141-148.