Application of the polymerase chain reaction to detect fowl adenoviruses

Ping Jiang, Davor Ojkic, T. Tuboly, Paul Huber, Éva Nagy

Research output: Article

26 Citations (Scopus)

Abstract

The possibility of using the polymerase chain reaction (PCR) for the detection of fowl adenoviruses (FAdV) was tested. The optimal reaction parameters were evaluated and defined for purified genomic DNA of type 8 fowl adenovirus (FAdV-8), and then the same conditions were applied for nucleic acid extracted from infected cells. One hundred picograms of purified viral DNA, or 250 FAdV-8-infected cells, were detected by ethidium bromide staining of the PCR products in agarose gels. The sensitivity was increased to 10 pg purified viral DNA, or 25 infected cells, when the PCR products were hybridized with a specific labeled probe. Several field isolates of FAdV and the CELO virus (FAdV serotype 1) could be amplified by the same primers and conditions, but the size of the amplicons was smaller than that for the FAdV-8 PCR product. Other avian viruses and uninfected cell cultures tested negative.

Original languageEnglish
Pages (from-to)124-128
Number of pages5
JournalCanadian Journal of Veterinary Research
Volume63
Issue number2
Publication statusPublished - ápr. 1999

Fingerprint

Adenoviridae
polymerase chain reaction
chickens
Polymerase Chain Reaction
Fowl adenovirus A
Viral DNA
DNA
Ethidium
viruses
Sepharose
Nucleic Acids
cells
agarose
nucleic acids
Cell Culture Techniques
Gels
serotypes
cell culture
Staining and Labeling
Viruses

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Application of the polymerase chain reaction to detect fowl adenoviruses. / Jiang, Ping; Ojkic, Davor; Tuboly, T.; Huber, Paul; Nagy, Éva.

In: Canadian Journal of Veterinary Research, Vol. 63, No. 2, 04.1999, p. 124-128.

Research output: Article

Jiang, Ping ; Ojkic, Davor ; Tuboly, T. ; Huber, Paul ; Nagy, Éva. / Application of the polymerase chain reaction to detect fowl adenoviruses. In: Canadian Journal of Veterinary Research. 1999 ; Vol. 63, No. 2. pp. 124-128.
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