Application of isotachophoresis in commercial capillary electrophoresis instrument using C4D and UV detection

Péter I. Koczka, Róbert Bodor, Marián Masár, A. Gáspár

Research output: Article

8 Citations (Scopus)

Abstract

In this work, we tested the applicability of a commercial CE instrument (Agilent) for capillary ITP (CITP). The fused silica capillaries were flushed with PVP solution before each sample injection to suppress the EOF. As a dual-detection mode, commercial capacitively coupled contactless conductivity detection and ultraviolet detectors were applied. The experiments showed that the detection gap of the capacitively coupled contactless conductivity detection limits the achievable LOD and the separation resolution when the analyte CITP zones are very narrow, therefore long (120 cm) CE capillary was used and it was largely filled with the sample solution. CITP analyses of several real samples (leather extract, red wine, juice, and fizzy drink) have been demonstrated. In peak mode of CITP when the zone of a chromophore analyte is positioned between nonchromophore zones, excellent sensitivity (in submicromolar concentration range) could be achieved by ultraviolet detection. The hazardous chromate in low concentration was determined in the aqueous extract of tanned leather.

Original languageEnglish
Pages (from-to)2384-2392
Number of pages9
JournalElectrophoresis
Volume37
Issue number17-18
DOIs
Publication statusPublished - szept. 1 2016

Fingerprint

Isotachophoresis
Inosine Triphosphate
Capillary electrophoresis
Capillary Electrophoresis
Leather
Ultraviolet detectors
Chromates
Wine
Fused silica
Chromophores
Silicon Dioxide
Limit of Detection
Injections
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Application of isotachophoresis in commercial capillary electrophoresis instrument using C4D and UV detection. / Koczka, Péter I.; Bodor, Róbert; Masár, Marián; Gáspár, A.

In: Electrophoresis, Vol. 37, No. 17-18, 01.09.2016, p. 2384-2392.

Research output: Article

Koczka, Péter I. ; Bodor, Róbert ; Masár, Marián ; Gáspár, A. / Application of isotachophoresis in commercial capillary electrophoresis instrument using C4D and UV detection. In: Electrophoresis. 2016 ; Vol. 37, No. 17-18. pp. 2384-2392.
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