Antisera to γ-aminobutyric acid. 1. Production and characterization using a new model system

A. J. Hodgson, B. Penke, A. Erdei, I. W. Chubb, P. Somogyi

Research output: Article

303 Citations (Scopus)

Abstract

Antisera to the amino acid γ-aminobutyric acid (GABA) have been developed with the aim of immunohistochemical visualization of neurons that use it as a neurotransmitter. GABA bound to bovine serum albumin was the immunogen. The reactivities of the sera to GABA and a variety of structurally related compounds were tested by coupling these compounds to nitrocellulose paper activated with polylysine and glutaraldehyde and incubating the paper with the unlabeled antibody enzyme method, thus simulating immunohistochemistry of tissue sections. The antisera did not react with L-glutamate, L-aspartate, D-aspartate, glycine, taurine, L-glutamine, L-lysine, L-threonine, L-alanine, α-aminobutyrate, β-aminobutyrate, putrescine, or δ-aminolevulinate. There was cross-reaction with γ-amino-β-hydroxybutyrate, 1-10%, and the homologous of GABA: β-alanine, 1-10%, δ-aminovalerate, ~ 10%, and ε-amino-caproate, ~ 10%. The antisera reacted slightly with the dipeptide γ-aminobutyrylleucine, but not carnosine or homocarnosine. Immunostaining of GABA was completely abolished by adsorption of the sera to GABA coupled to polyacrylamide beads by glutaraldehyde. The immunohistochemical model is simple, amino acids and peptides are bound in the same way as in aldehyde-fixed tissue and, in contrast to radioimmunoassay, it uses an immunohistochemical detection system. This method has enabled us to define the high specificity of anti-GABA sera and to use them in some novel ways. The model should prove useful in assessing the specificity of other antisera.

Original languageEnglish
Pages (from-to)229-239
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume33
Issue number3
Publication statusPublished - 1985

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Aminobutyrates
gamma-Aminobutyric Acid
Immune Sera
Glutaral
Alanine
Serum
Carnosine
Hydroxybutyrates
D-Aspartic Acid
Amino Acids
Polylysine
Putrescine
Collodion
Dipeptides
Taurine
Cross Reactions
Threonine
Bovine Serum Albumin
Glutamine
Aspartic Acid

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Antisera to γ-aminobutyric acid. 1. Production and characterization using a new model system. / Hodgson, A. J.; Penke, B.; Erdei, A.; Chubb, I. W.; Somogyi, P.

In: Journal of Histochemistry and Cytochemistry, Vol. 33, No. 3, 1985, p. 229-239.

Research output: Article

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