Antisense repression of StubGAL83 affects root and tuber development in potato

Ágnes Lovas, Andrea Bimbó, László Szabó, Z. Bánfalvi

Research output: Article

36 Citations (Scopus)

Abstract

StubGAL83 is a potato gene that encodes the β-subunit of a protein kinase complex similar to the yeast SNF1, and the mammalian AMPK complexes that are modulated by changes in the cellular AMP/ATP ratio and are important regulators of metabolic and stress responses. Here we show that the expression of StubGAL83 in potato foliage is much higher in the dark than in the light and can be repressed by metabolisable sugars in the dark. The amounts of StubGAL83 mRNA are higher in sink than in source leaves. To unravel the role of StubGAL83, transgenic potato plants expressing a part of the StubGAL83 cDNA in antisense orientation under the control of the constitutive CaMV35S promoter were generated. Northern analysis revealed a reduction up to 90-95% in StubGAL83 mRNA accumulation in leaves of seven lines. Five out of these seven lines exhibited a reduction of StubGAL83 mRNA levels also in root and tuber tissues. Independent on the type of repression, the transgenic lines showed a delay in rooting and an increased sensitivity to salt stress. The roots were stunted and possessed less pronounced tap roots than the controls albeit with different severity in the different transgenic lines. The root cells were smaller and some of them had irregular shape. Tuberisation of the antisense-StubGAL83 lines was delayed, the size of the tubers was reduced while the number of tubers per plant was increased. These results together suggest that StubGAL83 affects root and tuber development probably by altering the metabolic status of the leaves.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalPlant Journal
Volume33
Issue number1
DOIs
Publication statusPublished - jan. 2003

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Solanum tuberosum
tubers
potatoes
Messenger RNA
Plant Tubers
genetically modified organisms
leaves
Physiological Stress
AMP-Activated Protein Kinases
Genetically Modified Plants
Adenosine Monophosphate
tap roots
AMP-activated protein kinase
Protein Kinases
Complementary DNA
Salts
Adenosine Triphosphate
Yeasts
protein kinases
plant anatomy

ASJC Scopus subject areas

  • Plant Science

Cite this

Antisense repression of StubGAL83 affects root and tuber development in potato. / Lovas, Ágnes; Bimbó, Andrea; Szabó, László; Bánfalvi, Z.

In: Plant Journal, Vol. 33, No. 1, 01.2003, p. 139-147.

Research output: Article

Lovas, Ágnes ; Bimbó, Andrea ; Szabó, László ; Bánfalvi, Z. / Antisense repression of StubGAL83 affects root and tuber development in potato. In: Plant Journal. 2003 ; Vol. 33, No. 1. pp. 139-147.
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