Antioxidative, anti-inflammatory and hepatoprotective effects of resveratrol on oxidative stress-induced liver damage in tilapia (Oreochromis niloticus)

Rui Jia, Yao Li, Liping Cao, Jinliang Du, Tao Zheng, Hao Qian, Zhengyan Gu, G. Jeney, Pao Xu, Guojun Yin

Research output: Article

Abstract

Resveratrol, a dietary polyphenol, has been shown to exert antioxidation, hepatoprotection, anti-inflammation and immunostimulation. However, the effects and underlying mechanism of resveratrol on liver injury in fish are still unclear. In the present study, we investigated the potential protective effects and mechanism of resveratrol on oxidative stress-induced liver damage in tilapia. Fish were fed diet containing four doses of resveratrol (0, 0.1, 0.3, and 0.6 g/kg diet) for 60 days, and then given an intraperitoneal injection of H2O2 or saline. The results showed that administration of resveratrol significantly ameliorated H2O2-induced liver injury. In serum and liver, resveratrol treatment suppressed the oxidative stress, as evidenced by the decline of lipid peroxidation level and increase of antioxidant activity. Resveratrol also activated erythroid 2-related factor 2 (Nrf2) signaling pathway and enhanced the heme oxygenase 1 (HO-1), NAD(P) H:quinone oxidoreductase 1 (NQO-1), glutathione S-transferase (GST) mRNA levels. Meanwhile, resveratrol treatment repressed TLR2-Myd88-NF-κB signaling pathway to decrease the inflammatory response in H2O2-induced liver injury as evidenced by the lower interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-8 mRNA levels and higher IL-10 mRNA level. Moreover, resveratrol treatment attenuated immunotoxicity in liver of H2O2-treated fish, accompanied by upregulation of hepcidin (HEP), complement 3 (C3) and lysozyme (LZM) mRNA levels. Overall results suggested that the protection of resveratrol on H2O2-induced liver injury, inflammation and immunotoxicity was due to its antioxidant property and its ability to modulate the Nrf2 and TLR2-Myd88-NF-κB signaling pathways.

Original languageEnglish
Pages (from-to)56-66
Number of pages11
JournalComparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Volume215
DOIs
Publication statusPublished - jan. 1 2019

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Tilapia
Cichlids
Oxidative stress
Liver
Oxidative Stress
Anti-Inflammatory Agents
Fish
Messenger RNA
Fishes
Wounds and Injuries
Nutrition
Antioxidants
resveratrol
Hepcidins
Diet
Inflammation
Complement C3
Heme Oxygenase-1
Polyphenols
Muramidase

Keywords

    ASJC Scopus subject areas

    • Biochemistry
    • Physiology
    • Toxicology
    • Cell Biology
    • Health, Toxicology and Mutagenesis

    Cite this

    Antioxidative, anti-inflammatory and hepatoprotective effects of resveratrol on oxidative stress-induced liver damage in tilapia (Oreochromis niloticus). / Jia, Rui; Li, Yao; Cao, Liping; Du, Jinliang; Zheng, Tao; Qian, Hao; Gu, Zhengyan; Jeney, G.; Xu, Pao; Yin, Guojun.

    In: Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, Vol. 215, 01.01.2019, p. 56-66.

    Research output: Article

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    AU - Li, Yao

    AU - Cao, Liping

    AU - Du, Jinliang

    AU - Zheng, Tao

    AU - Qian, Hao

    AU - Gu, Zhengyan

    AU - Jeney, G.

    AU - Xu, Pao

    AU - Yin, Guojun

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