Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil

Anca Hermenean, Miruna Stan, Aurel Ardelean, Luminiţa Pilat, Ciprian Valentin Mihali, Cristina Popescu, Lajos Nagy, György Deák, Miklós Zsuga, Sándor Kéki, Ildikó Bácskay, Ferenc Fenyvesi, Marieta Costache, Anca Dinischiotu, Miklós Vecsernyés

Research output: Article

9 Citations (Scopus)


This study has assessed the protective efficacy of Silybum marianum seed oil (SMSO) in the context of CCl4-induced injury and oxidative stress in murine liver. Based on the GC-MS analysis, linoleic and stearic acids, tocopherol, ascorbic acid 2,6 dihexadecanoate and other constituents were identified in SMSO. Swiss mice received oral doses of SMSO daily for 21 days (10 g/kg b.w.) and subsequently injected i.p. with CCl4 (50% v/v in olive oil; 1 ml/kg) on the 22nd day. CCl4 administration induced an elevation of serum amino- and glutamyl transferases activities and an increased peroxidation, as well as a decrease of SOD, CAT, GPx, GR and GST activities in liver. SMSO successfully prevented oxidative stress and restored the biochemical parameters, hepatic architecture and expression of TNF-alpha. These findings suggest that SMSO was effective in counteracting the damaging effects of CCl4-induced injury in hepatocytes, probably due to its inherent antioxidant properties.

Original languageEnglish
Pages (from-to)147-158
Number of pages12
JournalOpen Life Sciences
Issue number1
Publication statusPublished - jan. 1 2015


ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Hermenean, A., Stan, M., Ardelean, A., Pilat, L., Mihali, C. V., Popescu, C., Nagy, L., Deák, G., Zsuga, M., Kéki, S., Bácskay, I., Fenyvesi, F., Costache, M., Dinischiotu, A., & Vecsernyés, M. (2015). Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil. Open Life Sciences, 10(1), 147-158.