A new method has been developed for the separation of reduced and oxidized forms of glutathione from amino acid mixtures. The samples were spotted on Kieselgel plates and developed in phenol-water = 7:3 containing SDS. The separation was performed in pressurized ultramicro chamber. The running time was shorter than that in a normal chamber, decreasing the possibility of diffusion. In addition to the two forms of glutathione, nine different kinds of amino acids could be separated. The method affords a possibility for rapid analysis of two forms of glutathione both in the clinical and industrial practice.
ASJC Scopus subject areas
- Molecular Medicine