Analysis of a novel RNA virus in a wild northern white-breasted hedgehog (Erinaceus roumanicus)

Gábor Reuter, Éva Várallyay, Dániel Baráth, Gábor Földvári, Sándor Szekeres, Ákos Boros, Beatrix Kapusinszky, Eric Delwart, Péter Pankovics

Research output: Article

Abstract

Tombusviruses are generally considered plant viruses. A novel tombus-/carmotetravirus-like RNA virus was identified in a faecal sample and blood and muscle tissues from a wild northern white-breasted hedgehog (Erinaceus roumanicus). The complete genome of the virus, called H14-hedgehog/2015/HUN (GenBank accession number MN044446), is 4,118 nucleotides in length with a readthrough stop codon of type/group 1 in ORF1 and lacks a poly(A) tract at the 3′ end. The predicted ORF1-RT (RdRp) and the capsid proteins had low (31-33%) amino acid sequence identity to unclassified tombus-/noda-like viruses (Hubei tombus-like virus 12 and Beihai noda-like virus 10), respectively, discovered recently in invertebrate animals. An in vivo experimental plant inoculation study showed that an in vitro-transcribed H14-hedgehog/2015/HUN viral RNA did not replicate in Nicotiana benthamiana, Chenopodium quinoa, or Chenopodium murale, the most susceptible hosts for plant-origin tombusviruses.

Original languageEnglish
Pages (from-to)3065-3071
Number of pages7
JournalArchives of virology
Volume164
Issue number12
DOIs
Publication statusPublished - dec. 1 2019

Fingerprint

Hedgehogs
RNA Viruses
Tombusvirus
Viruses
Chenopodium
Chenopodium quinoa
Plant Viruses
Poly A
Terminator Codon
Nucleic Acid Databases
Viral RNA
Capsid Proteins
Invertebrates
Tobacco
Amino Acid Sequence
Nucleotides
Genome
Muscles

ASJC Scopus subject areas

  • Virology

Cite this

Analysis of a novel RNA virus in a wild northern white-breasted hedgehog (Erinaceus roumanicus). / Reuter, Gábor; Várallyay, Éva; Baráth, Dániel; Földvári, Gábor; Szekeres, Sándor; Boros, Ákos; Kapusinszky, Beatrix; Delwart, Eric; Pankovics, Péter.

In: Archives of virology, Vol. 164, No. 12, 01.12.2019, p. 3065-3071.

Research output: Article

Reuter, Gábor ; Várallyay, Éva ; Baráth, Dániel ; Földvári, Gábor ; Szekeres, Sándor ; Boros, Ákos ; Kapusinszky, Beatrix ; Delwart, Eric ; Pankovics, Péter. / Analysis of a novel RNA virus in a wild northern white-breasted hedgehog (Erinaceus roumanicus). In: Archives of virology. 2019 ; Vol. 164, No. 12. pp. 3065-3071.
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