Abstract
Tombusviruses are generally considered plant viruses. A novel tombus-/carmotetravirus-like RNA virus was identified in a faecal sample and blood and muscle tissues from a wild northern white-breasted hedgehog (Erinaceus roumanicus). The complete genome of the virus, called H14-hedgehog/2015/HUN (GenBank accession number MN044446), is 4,118 nucleotides in length with a readthrough stop codon of type/group 1 in ORF1 and lacks a poly(A) tract at the 3′ end. The predicted ORF1-RT (RdRp) and the capsid proteins had low (31-33%) amino acid sequence identity to unclassified tombus-/noda-like viruses (Hubei tombus-like virus 12 and Beihai noda-like virus 10), respectively, discovered recently in invertebrate animals. An in vivo experimental plant inoculation study showed that an in vitro-transcribed H14-hedgehog/2015/HUN viral RNA did not replicate in Nicotiana benthamiana, Chenopodium quinoa, or Chenopodium murale, the most susceptible hosts for plant-origin tombusviruses.
| Original language | English |
|---|---|
| Pages (from-to) | 3065-3071 |
| Number of pages | 7 |
| Journal | Archives of virology |
| Volume | 164 |
| Issue number | 12 |
| DOIs | |
| Publication status | Published - dec. 1 2019 |
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ASJC Scopus subject areas
- Virology
Cite this
Analysis of a novel RNA virus in a wild northern white-breasted hedgehog (Erinaceus roumanicus). / Reuter, Gábor; Várallyay, Éva; Baráth, Dániel; Földvári, Gábor; Szekeres, Sándor; Boros, Ákos; Kapusinszky, Beatrix; Delwart, Eric; Pankovics, Péter.
In: Archives of virology, Vol. 164, No. 12, 01.12.2019, p. 3065-3071.Research output: Article
}
TY - JOUR
T1 - Analysis of a novel RNA virus in a wild northern white-breasted hedgehog (Erinaceus roumanicus)
AU - Reuter, Gábor
AU - Várallyay, Éva
AU - Baráth, Dániel
AU - Földvári, Gábor
AU - Szekeres, Sándor
AU - Boros, Ákos
AU - Kapusinszky, Beatrix
AU - Delwart, Eric
AU - Pankovics, Péter
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Tombusviruses are generally considered plant viruses. A novel tombus-/carmotetravirus-like RNA virus was identified in a faecal sample and blood and muscle tissues from a wild northern white-breasted hedgehog (Erinaceus roumanicus). The complete genome of the virus, called H14-hedgehog/2015/HUN (GenBank accession number MN044446), is 4,118 nucleotides in length with a readthrough stop codon of type/group 1 in ORF1 and lacks a poly(A) tract at the 3′ end. The predicted ORF1-RT (RdRp) and the capsid proteins had low (31-33%) amino acid sequence identity to unclassified tombus-/noda-like viruses (Hubei tombus-like virus 12 and Beihai noda-like virus 10), respectively, discovered recently in invertebrate animals. An in vivo experimental plant inoculation study showed that an in vitro-transcribed H14-hedgehog/2015/HUN viral RNA did not replicate in Nicotiana benthamiana, Chenopodium quinoa, or Chenopodium murale, the most susceptible hosts for plant-origin tombusviruses.
AB - Tombusviruses are generally considered plant viruses. A novel tombus-/carmotetravirus-like RNA virus was identified in a faecal sample and blood and muscle tissues from a wild northern white-breasted hedgehog (Erinaceus roumanicus). The complete genome of the virus, called H14-hedgehog/2015/HUN (GenBank accession number MN044446), is 4,118 nucleotides in length with a readthrough stop codon of type/group 1 in ORF1 and lacks a poly(A) tract at the 3′ end. The predicted ORF1-RT (RdRp) and the capsid proteins had low (31-33%) amino acid sequence identity to unclassified tombus-/noda-like viruses (Hubei tombus-like virus 12 and Beihai noda-like virus 10), respectively, discovered recently in invertebrate animals. An in vivo experimental plant inoculation study showed that an in vitro-transcribed H14-hedgehog/2015/HUN viral RNA did not replicate in Nicotiana benthamiana, Chenopodium quinoa, or Chenopodium murale, the most susceptible hosts for plant-origin tombusviruses.
UR - http://www.scopus.com/inward/record.url?scp=85073920258&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85073920258&partnerID=8YFLogxK
U2 - 10.1007/s00705-019-04414-7
DO - 10.1007/s00705-019-04414-7
M3 - Article
C2 - 31549303
AN - SCOPUS:85073920258
VL - 164
SP - 3065
EP - 3071
JO - Archives of Virology
JF - Archives of Virology
SN - 0304-8608
IS - 12
ER -