Two alkaliphilic and moderately halophilic bacterial strains B16-10 T and Z23-18 characterized by optimal growth at pH 9.0– 10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunsag National Park, Hungary. Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia. The isolates were facultative anaerobic, catalase positive, oxidase negative. Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone. Anteiso-C 15: 0 , C 16: 1 !11c and iso-C 14: 0 were the major cellular fatty acids. The DNA G+C content of both strains was 35.8 mol%. The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10 T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006 T (98.7 and 99.1 %), A. macyae JMM-4 T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6 T (97.7 and 98.4 %), A. alkalilacustris Z-0521 T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340 T (97.5 and 98.2 %). However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10 T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp. nov. is proposed. The type strain is B16-10 T (=DSM 29790 T =NCAIM B 02608 T ).
|Number of pages||7|
|Journal||International journal of systematic and evolutionary microbiology|
|Publication status||Published - márc. 2019|
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics