Anaerobacillus alkaliphilus sp. Nov., a novel alkaliphilic and moderately halophilic bacterium

Andrea K. Borsodi, Júlia M. Aszalós, Peter Bihari, Istvan Nagy, Peter Schumann, Cathrin Spröer, Attila L. Kovacs, Karoly Bóka, Peter Dobosy, Mihaly Óvari, Tibor Szili-Kovacs, Erika Tóth

Research output: Article

2 Citations (Scopus)

Abstract

Two alkaliphilic and moderately halophilic bacterial strains B16-10 T and Z23-18 characterized by optimal growth at pH 9.0– 10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunsag National Park, Hungary. Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia. The isolates were facultative anaerobic, catalase positive, oxidase negative. Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone. Anteiso-C 15: 0 , C 16: 1 !11c and iso-C 14: 0 were the major cellular fatty acids. The DNA G+C content of both strains was 35.8 mol%. The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10 T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006 T (98.7 and 99.1 %), A. macyae JMM-4 T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6 T (97.7 and 98.4 %), A. alkalilacustris Z-0521 T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340 T (97.5 and 98.2 %). However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10 T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp. nov. is proposed. The type strain is B16-10 T (=DSM 29790 T =NCAIM B 02608 T ).

Original languageEnglish
Pages (from-to)631-637
Number of pages7
JournalInternational journal of systematic and evolutionary microbiology
Volume69
Issue number3
DOIs
Publication statusPublished - márc. 2019

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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