Abstract
Loss of the Y chromosome in Drosophila has no impact on cell viability and therefore allows us to assay the impact of environmental agents and genetic alterations on chromosomal loss. To detect in vivo chromosome loss in cells of the developing Drosophila wing primordia, we first engineered a Y chromosome with an attP docking site. By making use of the FC31 integrase system, we site-specifically integrated a genomic transgene encompassing the multiple wing hair (mwh) locus into this attP site, leading to a mwh+Y chromosome. This chromosome fully rescues the mwh mutant phenotype, an excellent recessive wing cell marker mutation. Loss of this mwh+Y chromosome in wing primordial cells then leads to manifestation of the mwh mutant phenotype in mwh-homozygous cells. The forming mwh clones permit us to quantify the effect of agents and genetic alterations by assaying frequency and size of the mwh mosaic spots. To illustrate the use of the mwh+Y loss system, the effects of four known mutagens (X-rays, colchicine, ethyl methanesulfonate, and formaldehyde) and two genetic conditions (loss- and gain-of-function lodestar mutant alleles) are documented. The procedure is simple, sensitive, and inexpensive.
| Original language | English |
|---|---|
| Pages (from-to) | 1095-1102 |
| Number of pages | 8 |
| Journal | G3: Genes, Genomes, Genetics |
| Volume | 2 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - szept. 2012 |
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ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Genetics(clinical)
Cite this
An assay to detect in vivo y chromosome loss in drosophila wing disc cells. / Szabad, J.; Bellen, Hugo J.; Venken, Koen J T.
In: G3: Genes, Genomes, Genetics, Vol. 2, No. 9, 09.2012, p. 1095-1102.Research output: Article
}
TY - JOUR
T1 - An assay to detect in vivo y chromosome loss in drosophila wing disc cells
AU - Szabad, J.
AU - Bellen, Hugo J.
AU - Venken, Koen J T
PY - 2012/9
Y1 - 2012/9
N2 - Loss of the Y chromosome in Drosophila has no impact on cell viability and therefore allows us to assay the impact of environmental agents and genetic alterations on chromosomal loss. To detect in vivo chromosome loss in cells of the developing Drosophila wing primordia, we first engineered a Y chromosome with an attP docking site. By making use of the FC31 integrase system, we site-specifically integrated a genomic transgene encompassing the multiple wing hair (mwh) locus into this attP site, leading to a mwh+Y chromosome. This chromosome fully rescues the mwh mutant phenotype, an excellent recessive wing cell marker mutation. Loss of this mwh+Y chromosome in wing primordial cells then leads to manifestation of the mwh mutant phenotype in mwh-homozygous cells. The forming mwh clones permit us to quantify the effect of agents and genetic alterations by assaying frequency and size of the mwh mosaic spots. To illustrate the use of the mwh+Y loss system, the effects of four known mutagens (X-rays, colchicine, ethyl methanesulfonate, and formaldehyde) and two genetic conditions (loss- and gain-of-function lodestar mutant alleles) are documented. The procedure is simple, sensitive, and inexpensive.
AB - Loss of the Y chromosome in Drosophila has no impact on cell viability and therefore allows us to assay the impact of environmental agents and genetic alterations on chromosomal loss. To detect in vivo chromosome loss in cells of the developing Drosophila wing primordia, we first engineered a Y chromosome with an attP docking site. By making use of the FC31 integrase system, we site-specifically integrated a genomic transgene encompassing the multiple wing hair (mwh) locus into this attP site, leading to a mwh+Y chromosome. This chromosome fully rescues the mwh mutant phenotype, an excellent recessive wing cell marker mutation. Loss of this mwh+Y chromosome in wing primordial cells then leads to manifestation of the mwh mutant phenotype in mwh-homozygous cells. The forming mwh clones permit us to quantify the effect of agents and genetic alterations by assaying frequency and size of the mwh mosaic spots. To illustrate the use of the mwh+Y loss system, the effects of four known mutagens (X-rays, colchicine, ethyl methanesulfonate, and formaldehyde) and two genetic conditions (loss- and gain-of-function lodestar mutant alleles) are documented. The procedure is simple, sensitive, and inexpensive.
KW - chromosome loss
KW - Drosophila
KW - Hair
KW - Multiple wing
KW - Spots
KW - Wing mosaic
KW - Y chromosome
UR - http://www.scopus.com/inward/record.url?scp=84883199418&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883199418&partnerID=8YFLogxK
U2 - 10.1534/g3.112.002899
DO - 10.1534/g3.112.002899
M3 - Article
C2 - 22973547
AN - SCOPUS:84883199418
VL - 2
SP - 1095
EP - 1102
JO - G3 (Bethesda, Md.)
JF - G3 (Bethesda, Md.)
SN - 2160-1836
IS - 9
ER -