Algal cell response to laboratory-induced cadmium stress: a multimethod approach

Nadica Ivošević DeNardis, Jadranka Pečar Ilić, Ivica Ružić, Nives Novosel, Tea Mišić Radić, Andreas Weber, Damir Kasum, Zuzana Pavlinska, Ria Katalin Balogh, Bálint Hajdu, Alžbeta Marček Chorvátová, B. Gyurcsik

Research output: Article

1 Citation (Scopus)

Abstract

We examined the response of algal cells to laboratory-induced cadmium stress in terms of physiological activity, autonomous features (motility and fluorescence), adhesion dynamics, nanomechanical properties, and protein expression by employing a multimethod approach. We develop a methodology based on the generalized mathematical model to predict free cadmium concentrations in culture. We used algal cells of Dunaliella tertiolecta, which are widespread in marine and freshwater systems, as a model organism. Cell adaptation to cadmium stress is manifested through cell shape deterioration, slower motility, and an increase of physiological activity. No significant change in growth dynamics showed how cells adapt to stress by increasing active surface area against toxic cadmium in the culture. It was accompanied by an increase in green fluorescence (most likely associated with cadmium vesicular transport and/or beta-carotene production), while no change was observed in the red endogenous fluorescence (associated with chlorophyll). To maintain the same rate of chlorophyll emission, the cell adaptation response was manifested through increased expression of the identified chlorophyll-binding protein(s) that are important for photosynthesis. Since production of these proteins represents cell defence mechanisms, they may also signal the presence of toxic metal in seawater. Protein expression affects the cell surface properties and, therefore, the dynamics of the adhesion process. Cells behave stiffer under stress with cadmium, and thus, the initial attachment and deformation are slower. Physicochemical and structural characterizations of algal cell surfaces are of key importance to interpret, rationalize, and predict the behaviour and fate of the cell under stress in vivo.

Original languageEnglish
JournalEuropean Biophysics Journal
DOIs
Publication statusPublished - jan. 1 2019

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Cadmium
Fluorescence
Poisons
Chlorophyll
Chlorophyll Binding Proteins
Proteins
Surface Properties
Cell Shape
beta Carotene
Photosynthesis
Seawater
Fresh Water
Theoretical Models
Metals
Growth

Keywords

    ASJC Scopus subject areas

    • Biophysics

    Cite this

    Algal cell response to laboratory-induced cadmium stress : a multimethod approach. / Ivošević DeNardis, Nadica; Pečar Ilić, Jadranka; Ružić, Ivica; Novosel, Nives; Mišić Radić, Tea; Weber, Andreas; Kasum, Damir; Pavlinska, Zuzana; Balogh, Ria Katalin; Hajdu, Bálint; Marček Chorvátová, Alžbeta; Gyurcsik, B.

    In: European Biophysics Journal, 01.01.2019.

    Research output: Article

    Ivošević DeNardis, N, Pečar Ilić, J, Ružić, I, Novosel, N, Mišić Radić, T, Weber, A, Kasum, D, Pavlinska, Z, Balogh, RK, Hajdu, B, Marček Chorvátová, A & Gyurcsik, B 2019, 'Algal cell response to laboratory-induced cadmium stress: a multimethod approach' European Biophysics Journal. https://doi.org/10.1007/s00249-019-01347-6
    Ivošević DeNardis N, Pečar Ilić J, Ružić I, Novosel N, Mišić Radić T, Weber A et al. Algal cell response to laboratory-induced cadmium stress: a multimethod approach. European Biophysics Journal. 2019 jan. 1. https://doi.org/10.1007/s00249-019-01347-6
    Ivošević DeNardis, Nadica ; Pečar Ilić, Jadranka ; Ružić, Ivica ; Novosel, Nives ; Mišić Radić, Tea ; Weber, Andreas ; Kasum, Damir ; Pavlinska, Zuzana ; Balogh, Ria Katalin ; Hajdu, Bálint ; Marček Chorvátová, Alžbeta ; Gyurcsik, B. / Algal cell response to laboratory-induced cadmium stress : a multimethod approach. In: European Biophysics Journal. 2019.
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    abstract = "We examined the response of algal cells to laboratory-induced cadmium stress in terms of physiological activity, autonomous features (motility and fluorescence), adhesion dynamics, nanomechanical properties, and protein expression by employing a multimethod approach. We develop a methodology based on the generalized mathematical model to predict free cadmium concentrations in culture. We used algal cells of Dunaliella tertiolecta, which are widespread in marine and freshwater systems, as a model organism. Cell adaptation to cadmium stress is manifested through cell shape deterioration, slower motility, and an increase of physiological activity. No significant change in growth dynamics showed how cells adapt to stress by increasing active surface area against toxic cadmium in the culture. It was accompanied by an increase in green fluorescence (most likely associated with cadmium vesicular transport and/or beta-carotene production), while no change was observed in the red endogenous fluorescence (associated with chlorophyll). To maintain the same rate of chlorophyll emission, the cell adaptation response was manifested through increased expression of the identified chlorophyll-binding protein(s) that are important for photosynthesis. Since production of these proteins represents cell defence mechanisms, they may also signal the presence of toxic metal in seawater. Protein expression affects the cell surface properties and, therefore, the dynamics of the adhesion process. Cells behave stiffer under stress with cadmium, and thus, the initial attachment and deformation are slower. Physicochemical and structural characterizations of algal cell surfaces are of key importance to interpret, rationalize, and predict the behaviour and fate of the cell under stress in vivo.",
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    AU - Ivošević DeNardis, Nadica

    AU - Pečar Ilić, Jadranka

    AU - Ružić, Ivica

    AU - Novosel, Nives

    AU - Mišić Radić, Tea

    AU - Weber, Andreas

    AU - Kasum, Damir

    AU - Pavlinska, Zuzana

    AU - Balogh, Ria Katalin

    AU - Hajdu, Bálint

    AU - Marček Chorvátová, Alžbeta

    AU - Gyurcsik, B.

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    N2 - We examined the response of algal cells to laboratory-induced cadmium stress in terms of physiological activity, autonomous features (motility and fluorescence), adhesion dynamics, nanomechanical properties, and protein expression by employing a multimethod approach. We develop a methodology based on the generalized mathematical model to predict free cadmium concentrations in culture. We used algal cells of Dunaliella tertiolecta, which are widespread in marine and freshwater systems, as a model organism. Cell adaptation to cadmium stress is manifested through cell shape deterioration, slower motility, and an increase of physiological activity. No significant change in growth dynamics showed how cells adapt to stress by increasing active surface area against toxic cadmium in the culture. It was accompanied by an increase in green fluorescence (most likely associated with cadmium vesicular transport and/or beta-carotene production), while no change was observed in the red endogenous fluorescence (associated with chlorophyll). To maintain the same rate of chlorophyll emission, the cell adaptation response was manifested through increased expression of the identified chlorophyll-binding protein(s) that are important for photosynthesis. Since production of these proteins represents cell defence mechanisms, they may also signal the presence of toxic metal in seawater. Protein expression affects the cell surface properties and, therefore, the dynamics of the adhesion process. Cells behave stiffer under stress with cadmium, and thus, the initial attachment and deformation are slower. Physicochemical and structural characterizations of algal cell surfaces are of key importance to interpret, rationalize, and predict the behaviour and fate of the cell under stress in vivo.

    AB - We examined the response of algal cells to laboratory-induced cadmium stress in terms of physiological activity, autonomous features (motility and fluorescence), adhesion dynamics, nanomechanical properties, and protein expression by employing a multimethod approach. We develop a methodology based on the generalized mathematical model to predict free cadmium concentrations in culture. We used algal cells of Dunaliella tertiolecta, which are widespread in marine and freshwater systems, as a model organism. Cell adaptation to cadmium stress is manifested through cell shape deterioration, slower motility, and an increase of physiological activity. No significant change in growth dynamics showed how cells adapt to stress by increasing active surface area against toxic cadmium in the culture. It was accompanied by an increase in green fluorescence (most likely associated with cadmium vesicular transport and/or beta-carotene production), while no change was observed in the red endogenous fluorescence (associated with chlorophyll). To maintain the same rate of chlorophyll emission, the cell adaptation response was manifested through increased expression of the identified chlorophyll-binding protein(s) that are important for photosynthesis. Since production of these proteins represents cell defence mechanisms, they may also signal the presence of toxic metal in seawater. Protein expression affects the cell surface properties and, therefore, the dynamics of the adhesion process. Cells behave stiffer under stress with cadmium, and thus, the initial attachment and deformation are slower. Physicochemical and structural characterizations of algal cell surfaces are of key importance to interpret, rationalize, and predict the behaviour and fate of the cell under stress in vivo.

    KW - Adhesion kinetics

    KW - Autofluorescence

    KW - Cadmium bioavailability

    KW - Cell stress adaptation

    KW - Nanomechanics

    KW - Protein expression

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