Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum

Gábor Kohut, Brigitta Oláh, A. Ádám, Jorge García-Martínez, L. Hornok

Research output: Article

15 Citations (Scopus)

Abstract

A homologue of the adenylyl cyclase (AC) gene of Neurospora crassa, named Fpacy1 was cloned from the genomic library of Fusarium proliferatum ITEM 2287 by screening the library with a DNA fragment amplified by using PCR primers designed from conserved sequences of the catalytic domain of AC genes from other fungi. The deduced FPACY1 protein had 53-77% identity with the AC proteins of other fungi. ΔFpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increased conidiation and delayed conidial germination. Colonization capability of the mutants, assessed on maize seedlings and tomato fruits also was adversely affected. In sexual crosses the AC mutants retained full male fertility, but their female fertility decreased significantly. Disruption of Fpacy1 abolished vegetative self-incompatibility, suggesting that the AC gene is involved in multiple developmental processes related to vegetative growth, as well as sexual and parasexual events. The elevated thermo- and H2O2-tolerance of the ΔFpacy1 mutants was coupled to an increased sensitivity towards Cd and Cu, indicating that the cAMP signaling pathway may have both negative and positive regulatory roles on the stress response mechanisms of fungal cells. When grown under nitrogen limitation conditions, the ΔFpacy1 mutants produced an average of ~274 μg g-1 bikaverin, whereas only traces of this metabolite was detected in the wild type. This finding provides further evidence of the role of the cAMP-PKA pathway in regulating bikaverin production.

Original languageEnglish
Pages (from-to)59-71
Number of pages13
JournalJournal of Basic Microbiology
Volume50
Issue number1
DOIs
Publication statusPublished - febr. 2010

Fingerprint

Fusarium
Heavy Metals
Adenylyl Cyclases
Genes
Fertility
Fungi
Neurospora crassa
Genomic Library
Conserved Sequence
Lycopersicon esculentum
Growth
Germination
Seedlings
Libraries
Zea mays
Fruit
Catalytic Domain
Proteins
Nitrogen
bikaverin

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum. / Kohut, Gábor; Oláh, Brigitta; Ádám, A.; García-Martínez, Jorge; Hornok, L.

In: Journal of Basic Microbiology, Vol. 50, No. 1, 02.2010, p. 59-71.

Research output: Article

@article{f62f1e07b2fe41678ec61807724e04c6,
title = "Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum",
abstract = "A homologue of the adenylyl cyclase (AC) gene of Neurospora crassa, named Fpacy1 was cloned from the genomic library of Fusarium proliferatum ITEM 2287 by screening the library with a DNA fragment amplified by using PCR primers designed from conserved sequences of the catalytic domain of AC genes from other fungi. The deduced FPACY1 protein had 53-77{\%} identity with the AC proteins of other fungi. ΔFpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increased conidiation and delayed conidial germination. Colonization capability of the mutants, assessed on maize seedlings and tomato fruits also was adversely affected. In sexual crosses the AC mutants retained full male fertility, but their female fertility decreased significantly. Disruption of Fpacy1 abolished vegetative self-incompatibility, suggesting that the AC gene is involved in multiple developmental processes related to vegetative growth, as well as sexual and parasexual events. The elevated thermo- and H2O2-tolerance of the ΔFpacy1 mutants was coupled to an increased sensitivity towards Cd and Cu, indicating that the cAMP signaling pathway may have both negative and positive regulatory roles on the stress response mechanisms of fungal cells. When grown under nitrogen limitation conditions, the ΔFpacy1 mutants produced an average of ~274 μg g-1 bikaverin, whereas only traces of this metabolite was detected in the wild type. This finding provides further evidence of the role of the cAMP-PKA pathway in regulating bikaverin production.",
keywords = "Abiotic stress, Adenylyl cyclase, Development, Fusarium, Secondary metabolite",
author = "G{\'a}bor Kohut and Brigitta Ol{\'a}h and A. {\'A}d{\'a}m and Jorge Garc{\'i}a-Mart{\'i}nez and L. Hornok",
year = "2010",
month = "2",
doi = "10.1002/jobm.200900113",
language = "English",
volume = "50",
pages = "59--71",
journal = "Journal of Basic Microbiology",
issn = "0233-111X",
publisher = "Wiley-VCH Verlag",
number = "1",

}

TY - JOUR

T1 - Adenylyl cyclase regulates heavy metal sensitivity, bikaverin production and plant tissue colonization in Fusarium proliferatum

AU - Kohut, Gábor

AU - Oláh, Brigitta

AU - Ádám, A.

AU - García-Martínez, Jorge

AU - Hornok, L.

PY - 2010/2

Y1 - 2010/2

N2 - A homologue of the adenylyl cyclase (AC) gene of Neurospora crassa, named Fpacy1 was cloned from the genomic library of Fusarium proliferatum ITEM 2287 by screening the library with a DNA fragment amplified by using PCR primers designed from conserved sequences of the catalytic domain of AC genes from other fungi. The deduced FPACY1 protein had 53-77% identity with the AC proteins of other fungi. ΔFpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increased conidiation and delayed conidial germination. Colonization capability of the mutants, assessed on maize seedlings and tomato fruits also was adversely affected. In sexual crosses the AC mutants retained full male fertility, but their female fertility decreased significantly. Disruption of Fpacy1 abolished vegetative self-incompatibility, suggesting that the AC gene is involved in multiple developmental processes related to vegetative growth, as well as sexual and parasexual events. The elevated thermo- and H2O2-tolerance of the ΔFpacy1 mutants was coupled to an increased sensitivity towards Cd and Cu, indicating that the cAMP signaling pathway may have both negative and positive regulatory roles on the stress response mechanisms of fungal cells. When grown under nitrogen limitation conditions, the ΔFpacy1 mutants produced an average of ~274 μg g-1 bikaverin, whereas only traces of this metabolite was detected in the wild type. This finding provides further evidence of the role of the cAMP-PKA pathway in regulating bikaverin production.

AB - A homologue of the adenylyl cyclase (AC) gene of Neurospora crassa, named Fpacy1 was cloned from the genomic library of Fusarium proliferatum ITEM 2287 by screening the library with a DNA fragment amplified by using PCR primers designed from conserved sequences of the catalytic domain of AC genes from other fungi. The deduced FPACY1 protein had 53-77% identity with the AC proteins of other fungi. ΔFpacy1 mutants obtained by targeted gene disruption showed retarded vegetative growth, increased conidiation and delayed conidial germination. Colonization capability of the mutants, assessed on maize seedlings and tomato fruits also was adversely affected. In sexual crosses the AC mutants retained full male fertility, but their female fertility decreased significantly. Disruption of Fpacy1 abolished vegetative self-incompatibility, suggesting that the AC gene is involved in multiple developmental processes related to vegetative growth, as well as sexual and parasexual events. The elevated thermo- and H2O2-tolerance of the ΔFpacy1 mutants was coupled to an increased sensitivity towards Cd and Cu, indicating that the cAMP signaling pathway may have both negative and positive regulatory roles on the stress response mechanisms of fungal cells. When grown under nitrogen limitation conditions, the ΔFpacy1 mutants produced an average of ~274 μg g-1 bikaverin, whereas only traces of this metabolite was detected in the wild type. This finding provides further evidence of the role of the cAMP-PKA pathway in regulating bikaverin production.

KW - Abiotic stress

KW - Adenylyl cyclase

KW - Development

KW - Fusarium

KW - Secondary metabolite

UR - http://www.scopus.com/inward/record.url?scp=77049128587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77049128587&partnerID=8YFLogxK

U2 - 10.1002/jobm.200900113

DO - 10.1002/jobm.200900113

M3 - Article

VL - 50

SP - 59

EP - 71

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

SN - 0233-111X

IS - 1

ER -