Additive effect of 5-HT2C and CB1 receptor blockade on the regulation of sleep-wake cycle

Emese Bogáthy, Noémi Papp, L. Tóthfalusi, Szilvia Vas, György Bagdy

Research output: Article

Abstract

Background: Previous data show that serotonin 2C (5-HT 2C ) and cannabinoid 1 (CB 1 ) receptors have a role in the modulation of sleep-wake cycle. Namely, antagonists on these receptors promoted wakefulness and inhibited rapid eye movement sleep (REMS) in rodents. The interaction of these receptors are also present in other physiological functions, such as the regulation of appetite. Blockade of 5-HT 2C receptors modulat the effect of CB 1 receptor antagonist, presumably in consecutive or interdependent steps. Here we investigate, whether previous blockade of 5-HT 2C receptors can affect CB 1 receptor functions in the sleep-wake regulation. Results: Wistar rats were equipped with electroencephalography (EEG) and electromyography (EMG) electrodes. Following the recovery and habituation after surgery, animals were injected intraperitoneally (ip.) with SB-242084, a 5-HT 2C receptor antagonist (1.0 mg/kg) at light onset (beginning of passive phase) followed by an injection with AM-251, a CB 1 receptor antagonist (5.0 or 10.0 mg/kg, ip.) 10 min later. EEG, EMG and motor activity were analyzed for the subsequent 2 h. Both SB-242084 and AM-251 increased the time spent in active wakefulness, while decreased the time spent in non-REMS and REMS stages in the first 2 h of passive phase. In combination, the effect of the agents were additive, furthermore, statistical analysis did not show any interaction between the effects of these drugs in the modulation of vigilance stages. Conclusions: Our results suggest that 5-HT 2C receptor blockade followed by blockade of CB 1 receptors evoked additive effect on the regulation of sleep-wake pattern.

Original languageEnglish
Article number14
JournalBMC neuroscience
Volume20
Issue number1
DOIs
Publication statusPublished - márc. 20 2019

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Receptor, Serotonin, 5-HT2C
Cannabinoid Receptor CB1
Sleep
Cannabinoid Receptors
Cannabinoid Receptor Antagonists
Wakefulness
REM Sleep
Electromyography
Electroencephalography
Serotonin
Appetite Regulation
Sleep Stages
Eye Movements
Wistar Rats
Rodentia
Electrodes
Motor Activity
Light
Injections
Pharmaceutical Preparations

Keywords

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Cellular and Molecular Neuroscience

    Cite this

    Additive effect of 5-HT2C and CB1 receptor blockade on the regulation of sleep-wake cycle. / Bogáthy, Emese; Papp, Noémi; Tóthfalusi, L.; Vas, Szilvia; Bagdy, György.

    In: BMC neuroscience, Vol. 20, No. 1, 14, 20.03.2019.

    Research output: Article

    Bogáthy, Emese ; Papp, Noémi ; Tóthfalusi, L. ; Vas, Szilvia ; Bagdy, György. / Additive effect of 5-HT2C and CB1 receptor blockade on the regulation of sleep-wake cycle. In: BMC neuroscience. 2019 ; Vol. 20, No. 1.
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    AU - Bogáthy, Emese

    AU - Papp, Noémi

    AU - Tóthfalusi, L.

    AU - Vas, Szilvia

    AU - Bagdy, György

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    KW - Cannabinoid 1 receptor

    KW - Electroencephalography

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    KW - Serotonin 2C receptor

    KW - Sleep

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