Adaptation to tert-butyl hydroperoxide at a plasma membrane level in the fission yeast Schizosaccharomyces pombe parental strain and its t-BuOOH-resistant mutant

Nikoletta Kálmán, Zoltán Gazdag, Milan Čertík, József Belágyi, Samy A. Selim, István Pócsi, Miklós Pesti

Research output: Article

5 Citations (Scopus)

Abstract

The one-gene mutant hyd1-190 of the fission yeast Schizosaccharomyces pombe displayed four-fold resistance to tert-butyl hydroperoxide (t-BuOOH) in comparison with its parental strain hyd+. The cells of hyd1-190 exhibited a quantitative alteration in the sterol content and hence in the fatty acid composition of the plasma membrane, reflected in a two-fold amphotericin B sensitivity, increased rigidity of the plasma membrane, revealed by an elevated (Δ7.9°C) phase-transition temperature, measured by means of electron paramagnetic resonance spectroscopy, and a significantly decreased uptake of glycerol. Treatment of the strains with a subinhibitory concentration (0.2mM) of t-BuOOH induced adaptation via modification of the sterol and fatty acid compositions, resulting in increased (Δ3.95°C) and decreased (Δ6.83°C) phase-transition temperatures of the hyd+ and hyd1-190 strains, respectively, in order to defend the cells against the consequences of t-BuOOH-induced external oxidative stress. However, in contrast with hyd+, hyd1-190 lacks the ability to adapt to t-BuOOH at a cell level.

Original languageEnglish
Pages (from-to)215-225
Number of pages11
JournalJournal of basic microbiology
Volume54
Issue number3
DOIs
Publication statusPublished - márc. 1 2014

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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