Activation of mitochondrial ATP-sensitive potassium channels prevents neuronal cell death after ischemia in neonatal rats

Nishadi Rajapakse, Katsuyoshi Shimizu, Béla Kis, James Snipes, Z. Lacza, David Busija

Research output: Article

54 Citations (Scopus)

Abstract

Activation of mitochondrial ATP-sensitive potassium channels (mKATP) has been shown to protect against cell death following ischemia/reperfusion in the heart but not in brain. We examined whether mKATP activation with diazoxide (DIZ) prevents neuronal cell death following hypoxia-ischemia (HI) in 7-day-old rat pups. Rat pups were subjected to HI (left carotid ligation; 8% O2; 2.5 h), following administration of vehicle, 1.9 mg/kg DIZ, 3.8 mg/kg DIZ or DIZ plus 10 mg/kg 5-hydroxydecanoic acid (mKATP antagonist). Total infarct volume was reduced from 99.8±2.7% in vehicle animals to 80.6±4.2% in 3.8 mg/kg DIZ treated animals (n=85, PATP subunits concentrated in mitochondria. Fluorescent studies indicated DIZ directly depolarized the mitochondria. In conclusion, selective opening of mKATP prior to HI results in neuroprotection in immature rats.

Original languageEnglish
Pages (from-to)208-212
Number of pages5
JournalNeuroscience Letters
Volume327
Issue number3
DOIs
Publication statusPublished - júl. 26 2002

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Diazoxide
KATP Channels
Cell Death
Ischemia
Mitochondria
Reperfusion
Ligation
Brain
Hypoxia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activation of mitochondrial ATP-sensitive potassium channels prevents neuronal cell death after ischemia in neonatal rats. / Rajapakse, Nishadi; Shimizu, Katsuyoshi; Kis, Béla; Snipes, James; Lacza, Z.; Busija, David.

In: Neuroscience Letters, Vol. 327, No. 3, 26.07.2002, p. 208-212.

Research output: Article

Rajapakse, Nishadi ; Shimizu, Katsuyoshi ; Kis, Béla ; Snipes, James ; Lacza, Z. ; Busija, David. / Activation of mitochondrial ATP-sensitive potassium channels prevents neuronal cell death after ischemia in neonatal rats. In: Neuroscience Letters. 2002 ; Vol. 327, No. 3. pp. 208-212.
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