Activation of deoxycytidine kinase by various nucleoside analogues

Tatjana Spasokoukotskaja, Maria Sasvári-Székely, Lehel Hullán, Freidoun Albertioni, Staffan Eriksson, Maria Staub

Research output: Article

14 Citations (Scopus)

Abstract

The effect of different nucleoside analogues on deoxycytidine kinase (dCK) and thymidine kinase (TK) was compared in normal human lymphocytes and various leukemic cell lines. G-phase enriched tonsilar lymphocyte subpopulation treated by CdA showed more profound stimulation of dCK activity than S-phase cells. No substantial changes in TK activity were detected. CdA treatment increased the activity of dCK 4-fold in peripheral blood mononuclear cells (PBMC) and 2-fold in promyelocytic cell line HL60, too. However, no significant stimulation was detected either in CCRF-CEM or in K562 cell lines. 2-Cl-2'deoxy-2'F-adenine arabinoside (CAFdA), 2F-adenine arabinoside (F-araA) and cytosine arabinoside (AraC) had the same effect as CdA, although higher concentrations were needed for maximal activation. In contrast, treatment by dCyd caused slight inhibition of dCK. The possibility of interference of nucleoside analogues with the mechanisms of posttranslational modification of dCK was proposed.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalAdvances in experimental medicine and biology
Volume431
DOIs
Publication statusPublished - 1998

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Activation of deoxycytidine kinase by various nucleoside analogues'. Together they form a unique fingerprint.

  • Cite this