Acquired qnrVC1 and blaNDM-1 resistance markers in an international high-risk Pseudomonas aeruginosa ST773 clone

B. Kocsis, A. Tóth, Daniel Gulyas, Balazs Ligeti, Katalin Katona, Laszlo Rokusz, D. Szabó

Research output: Article

Abstract

A multidrug-resistant Pseudomonas aeruginosa PS1 isolated from urine clinical sample was investigated in this study. The strain exhibited resistance to piperacillin/tazobactam, ciprofloxacin, imipenem, ceftazidime but it was susceptible to colistin. Analysis of whole-genome sequencing data by ResFinder detected various resistance determinants including qnrVC1 and blaNDM-1. The multiresistant P. aeruginosa isolate belonged to ST773 high-risk clone. The qnrVC1 and blaNDM-1 determinants were incorporated into different integrons. Expression of blaNDM-1 was fourfold and qnrVC1 was twofold increased, compared to that of rpsL housekeeping gene. Mutations in gyrA Thr83Leu and parC Ser87Leu were detected and additionally qnrVC1 expression indicates protective effect of QnrVC1 pentapeptid protein on gyrase and topoisomerase. High-risk P. aeruginosa clones integrate various carbapenemase and other resistance determinants into their genomes that facilitates further dissemination of multiresistance among clinical isolates. We report blaNDM-1 and qnrVC1 genes in P. aeruginosa ST773 international high-risk clone.

Original languageEnglish
Pages (from-to)336-338
Number of pages3
JournalJournal of medical microbiology
Volume68
Issue number3
DOIs
Publication statusPublished - márc. 1 2019

Fingerprint

Pseudomonas aeruginosa
Clone Cells
Genome
Integrons
Colistin
Ceftazidime
Imipenem
Essential Genes
Ciprofloxacin
Urine
Mutation
Genes
Proteins

Keywords

    ASJC Scopus subject areas

    • Microbiology
    • Microbiology (medical)

    Cite this

    Acquired qnrVC1 and blaNDM-1 resistance markers in an international high-risk Pseudomonas aeruginosa ST773 clone. / Kocsis, B.; Tóth, A.; Gulyas, Daniel; Ligeti, Balazs; Katona, Katalin; Rokusz, Laszlo; Szabó, D.

    In: Journal of medical microbiology, Vol. 68, No. 3, 01.03.2019, p. 336-338.

    Research output: Article

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    AU - Ligeti, Balazs

    AU - Katona, Katalin

    AU - Rokusz, Laszlo

    AU - Szabó, D.

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