A two year old, bilaterally cryptorchid Hungarian halfbreed horse struck by lightning was dissected. Post mortem findings included the current marks, arboreal patterns of the pilo-erection at the gluteal region of the horse (Figure 1), acute vasodilatation, -hemorrhages, -edema in the subcutaneous tissue at same region (Figure 2); multiple hemorrhages in the sceletal muscles, in the myocardial tissues, and in the mucous membrane of the stomach, in the pancreas, in the lungs (Figure 3), in the kidneys; acute diffuse lungs edema (Figure 4); multiple subperitoneal-, subpleural-, subepi- (Figure 5), and subendocardial hemorrhages; acute leptomeningeal-, and brain congestion, -edema and hemorrhages; fracture of the 7th cervical vertebra. During the histopathological analysis they detected in the skin (at the entrance of the lightning): abnormal cleft between the corium and epidermis, vacuolisation/cytolysis of the keratinocytes (Figure 6), dilated capillaries with enlarged endothelial cells in the corium, acute interstitial hemorrhages, vacuolisated myofibers of the arredor pili muscle arranged perpendicularly on the epidermal layer (Figure 7), vacuolisated matrix cells of the dermal hair papilla (Figure 8), fragmentation of the collagen fibers in the corium (Figure 9); in the subcutis partial coagulation of the myofibers due to lightning strike (Figure 10); in the myocardium acute interstitial edema, hemorrhages and degeneration/vacuolisation of the Purkinje cells (Figure 11), and vacuolisation of media layer of the coronary arteries (Figure 12); acute perivascular erythrocytadiapedesis, -congestion, and edema in the brain and leptomeninges (Figure 13). The samples from the skin, subcutaneous tissue, myocardium and brain were examined immunohistochemically. For the primary antibody: claudin-5 (1:100; Zymed), Ki-67 (1:100; DAKO) were used. Immunohistochemical staining was performed using the streptavidin-peroxidase procedure: antigen-bound primary antibody was detected using standard avidin-biotin immunoperoxidase complex (DAKO LSAB2 Kit), the chromogen substrate was 3,3-diamino-benzidine tetrahydrochloride (DAB substrate-chromogen; DAKO, Denmark). Mayer's hemalaun was used for counter-staining. The endothelial cells of the different vessels of the samples showed intense membrane positivity for humanized anti-claudin-5 antibody. The authors did not detect the deletion of the claudin-5 protein in the vessels of different samples. The claudin-5 protein helped the detection of the extravasated (claudin-5 negative) erythrocytes around the (claudin-5 positive) vessels in the brain (Figure 14), leptomeninges (Figure 15), myocardium and skin.
|Number of pages||11|
|Journal||Magyar Allatorvosok Lapja|
|Publication status||Published - dec. 1 2008|
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