A somatostatin analogue induces translocation of Ku 86 autoantigen from the cytosol to the nucleus in colon tumour cells

József Tóvári, Béla Szende, József Bocsi, Arturo Falaschi, András Simoncsits, Sándor Pongor, Judit Érchegyi, Attila Steták, György Kéri

Research output: Article

22 Citations (Scopus)

Abstract

Flow cytometric and electron microscopic immunocytochemical studies have been performed in HT-29 human colon tumor cells in vitro, to determine and localise p86 Ku protein, which is a regulatory subunit of DNA-dependent kinase and a specific binding site for somatostatin. We have demonstrated that HT-29 cells contain p86 Ku and that the distribution between the cytoplasm and the nucleus is even. After administration of the somatostatin analogues Sandostatin and TT-232 to HT-29 cells, the p86 Ku content of the cytoplasmic compartment decreased in the first 4 h. An increase in the content of this protein in the nuclear compartment was observed at hour 1 followed by a decrease at hour 4 after treatment. Quantitative differences between the two analogues have been observed in this respect. The practical significance of these findings is discussed.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalCellular Signalling
Volume10
Issue number4
DOIs
Publication statusPublished - ápr. 1998

ASJC Scopus subject areas

  • Cell Biology

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