A simple standard technique for labyrinthectomy in the rat

A methodical communication with a detailed description of the surgical process

G. Nádasy, G. Raffai, E. Fehér, G. Schaming, E. Monos

Research output: Article

2 Citations (Scopus)

Abstract

Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

Original languageEnglish
Pages (from-to)354-360
Number of pages7
JournalPhysiology International
Volume103
Issue number3
DOIs
Publication statusPublished - szept. 1 2016

Fingerprint

Neuronal Plasticity
Fascia
Blister
Communication
Organ of Corti
Muscles
Mastoid
Cochlea
Facial Nerve
Deafness
Middle Ear
Inner Ear
Tendons
Drinking
Rodentia
Ethanol
Eating
Skin
sodium arsenite

ASJC Scopus subject areas

  • Physiology (medical)

Cite this

@article{9660a986b30f4b53b2b8022f37abd249,
title = "A simple standard technique for labyrinthectomy in the rat: A methodical communication with a detailed description of the surgical process",
abstract = "Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).",
keywords = "Experimental surgery, Labyrinthectomy, Neural plasticity, Rat, Vestibular organ",
author = "G. N{\'a}dasy and G. Raffai and E. Feh{\'e}r and G. Schaming and E. Monos",
year = "2016",
month = "9",
day = "1",
doi = "10.1556/2060.103.2016.3.8",
language = "English",
volume = "103",
pages = "354--360",
journal = "Physiology International",
issn = "2498-602X",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - A simple standard technique for labyrinthectomy in the rat

T2 - A methodical communication with a detailed description of the surgical process

AU - Nádasy, G.

AU - Raffai, G.

AU - Fehér, E.

AU - Schaming, G.

AU - Monos, E.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

AB - Aims: Labyrinthectomized rats are suitable models to test consequences of vestibular lesion and are widely used to study neural plasticity. We describe a combined microsurgical-chemical technique that can be routinely performed with minimum damage. Methods: Caudal leaflet of the parotis is elevated. The tendinous fascia covering the bulla is opened frontally from the sternomastoid muscle's tendon while sparing facial nerve branches. A 4 mm diameter hole is drilled into the bulla's hind lower lateral wall to open the common (in rodents) mastoid-tympanic cavity. The cochlear crista (promontory) at the lower posterior part of its medial wall is identified as a bony prominence. A 1 mm diameter hole is drilled into its lower part. The perilymphatic/endolymphatic fluids with tissue debris of the Corti organ are suctioned. Ethanol is injected into the hole. Finally, 10 μL of sodium arsenite solution (50 μM/mL) is pumped into the labyrinth and left in place for 15 min. Simple closure in two layers (fascia and skin) is sufficient. Results and conclusion: All rats had neurological symptoms specific for labyrinthectomy (muscle tone, body position, rotatory movements, nystagmus, central deafness). Otherwise, their behavior was unaffected, drinking and eating normally. After a few days, they learned to balance relying on visual and somatic stimuli (neuroplasticity).

KW - Experimental surgery

KW - Labyrinthectomy

KW - Neural plasticity

KW - Rat

KW - Vestibular organ

UR - http://www.scopus.com/inward/record.url?scp=85015868008&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015868008&partnerID=8YFLogxK

U2 - 10.1556/2060.103.2016.3.8

DO - 10.1556/2060.103.2016.3.8

M3 - Article

VL - 103

SP - 354

EP - 360

JO - Physiology International

JF - Physiology International

SN - 2498-602X

IS - 3

ER -