A simple method for monitoring the cysteine content in synthetic peptides

Kata Horváti, Szilvia Bösze, Ferenc Hudecz, Hedvig Medzihradszky-Schweiger

Research output: Article

6 Citations (Scopus)

Abstract

A new method for the determination of the cysteine content of synthetic peptides is described. For this purpose the classical amino acid analysis is not suitable, as cysteine may undergo oxidative dimerisation to cystine during the hydrolysis or evaporation. The intact peptide was reacted with N-ethylmaleimide (NEM) yielding a stable S-(N-ethylsuccinimido)-cysteine derivative, which could be separated by RP-HPLC and characterised by mass spectrometry. For the quantitative determination less than 0.1 μM peptide was sufficient.

Original languageEnglish
Pages (from-to)838-844
Number of pages7
JournalJournal of Peptide Science
Volume14
Issue number7
DOIs
Publication statusPublished - júl. 1 2008

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ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

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