A novel transgenic rabbit model with reduced repolarization reserve: Long QT syndrome caused by a dominant-negative mutation of the KCNE1 gene

Péter Major, I. Baczkó, László Hiripi, Katja E. Odening, Viktor Juhász, Zsófia Kohajda, András Horváth, György Seprényi, Mária Kovács, L. Virág, N. Jost, János Prorok, Balázs Ördög, Zoltán Doleschall, Stanley Nattel, A. Varró, Z. Bösze

Research output: Article

15 Citations (Scopus)

Abstract

Background and purpose The reliable assessment of proarrhythmic risk of compounds under development remains an elusive goal. Current safety guidelines focus on the effects of blocking the KCNH2/HERG ion channel-in tissues and animals with intact repolarization. Novel models with better predictive value are needed that more closely reflect the conditions in patients with cardiac remodelling and reduced repolarization reserve. Experimental Approach We have developed a model for the long QT syndrome type-5 in rabbits (LQT5) with cardiac-specific overexpression of a mutant (G52R) KCNE1 β-subunit of the channel that carries the slow delayed-rectifier K+-current (IKs). ECG parameters, including short-term variability of the QT interval (STVQT), a biomarker for proarrhythmic risk, and arrhythmia development were recorded. In vivo, arrhythmia susceptibility was evaluated by i.v. administration of the IKr blocker dofetilide. K+ currents were measured with the patch-clamp technique. Key Results Patch-clamp studies in ventricular myocytes isolated from LQT5 rabbits revealed accelerated IKs and IKr deactivation kinetics. At baseline, LQT5 animals exhibited slightly but significantly prolonged heart-rate corrected QT index (QTi) and increased STVQT. Dofetilide provoked Torsade-de-Pointes arrhythmia in a greater proportion of LQT5 rabbits, paralleled by a further increase in STVQT. Conclusion and Implications We have created a novel transgenic LQT5 rabbit model with increased susceptibility to drug-induced arrhythmias that may represent a useful model for testing proarrhythmic potential and for investigations of the mechanisms underlying arrhythmias and sudden cardiac death due to repolarization disturbances.

Original languageEnglish
Pages (from-to)2046-2061
Number of pages16
JournalBritish Journal of Pharmacology
Volume173
Issue number12
DOIs
Publication statusPublished - jún. 1 2016

Fingerprint

Long QT Syndrome
Rabbits
Mutation
Cardiac Arrhythmias
Genes
Torsades de Pointes
Sudden Cardiac Death
Patch-Clamp Techniques
Ion Channels
Muscle Cells
Electrocardiography
Biomarkers
Heart Rate
Guidelines
Safety

ASJC Scopus subject areas

  • Pharmacology

Cite this

A novel transgenic rabbit model with reduced repolarization reserve : Long QT syndrome caused by a dominant-negative mutation of the KCNE1 gene. / Major, Péter; Baczkó, I.; Hiripi, László; Odening, Katja E.; Juhász, Viktor; Kohajda, Zsófia; Horváth, András; Seprényi, György; Kovács, Mária; Virág, L.; Jost, N.; Prorok, János; Ördög, Balázs; Doleschall, Zoltán; Nattel, Stanley; Varró, A.; Bösze, Z.

In: British Journal of Pharmacology, Vol. 173, No. 12, 01.06.2016, p. 2046-2061.

Research output: Article

Major, P, Baczkó, I, Hiripi, L, Odening, KE, Juhász, V, Kohajda, Z, Horváth, A, Seprényi, G, Kovács, M, Virág, L, Jost, N, Prorok, J, Ördög, B, Doleschall, Z, Nattel, S, Varró, A & Bösze, Z 2016, 'A novel transgenic rabbit model with reduced repolarization reserve: Long QT syndrome caused by a dominant-negative mutation of the KCNE1 gene', British Journal of Pharmacology, vol. 173, no. 12, pp. 2046-2061. https://doi.org/10.1111/bph.13500
Major, Péter ; Baczkó, I. ; Hiripi, László ; Odening, Katja E. ; Juhász, Viktor ; Kohajda, Zsófia ; Horváth, András ; Seprényi, György ; Kovács, Mária ; Virág, L. ; Jost, N. ; Prorok, János ; Ördög, Balázs ; Doleschall, Zoltán ; Nattel, Stanley ; Varró, A. ; Bösze, Z. / A novel transgenic rabbit model with reduced repolarization reserve : Long QT syndrome caused by a dominant-negative mutation of the KCNE1 gene. In: British Journal of Pharmacology. 2016 ; Vol. 173, No. 12. pp. 2046-2061.
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abstract = "Background and purpose The reliable assessment of proarrhythmic risk of compounds under development remains an elusive goal. Current safety guidelines focus on the effects of blocking the KCNH2/HERG ion channel-in tissues and animals with intact repolarization. Novel models with better predictive value are needed that more closely reflect the conditions in patients with cardiac remodelling and reduced repolarization reserve. Experimental Approach We have developed a model for the long QT syndrome type-5 in rabbits (LQT5) with cardiac-specific overexpression of a mutant (G52R) KCNE1 β-subunit of the channel that carries the slow delayed-rectifier K+-current (IKs). ECG parameters, including short-term variability of the QT interval (STVQT), a biomarker for proarrhythmic risk, and arrhythmia development were recorded. In vivo, arrhythmia susceptibility was evaluated by i.v. administration of the IKr blocker dofetilide. K+ currents were measured with the patch-clamp technique. Key Results Patch-clamp studies in ventricular myocytes isolated from LQT5 rabbits revealed accelerated IKs and IKr deactivation kinetics. At baseline, LQT5 animals exhibited slightly but significantly prolonged heart-rate corrected QT index (QTi) and increased STVQT. Dofetilide provoked Torsade-de-Pointes arrhythmia in a greater proportion of LQT5 rabbits, paralleled by a further increase in STVQT. Conclusion and Implications We have created a novel transgenic LQT5 rabbit model with increased susceptibility to drug-induced arrhythmias that may represent a useful model for testing proarrhythmic potential and for investigations of the mechanisms underlying arrhythmias and sudden cardiac death due to repolarization disturbances.",
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T1 - A novel transgenic rabbit model with reduced repolarization reserve

T2 - Long QT syndrome caused by a dominant-negative mutation of the KCNE1 gene

AU - Major, Péter

AU - Baczkó, I.

AU - Hiripi, László

AU - Odening, Katja E.

AU - Juhász, Viktor

AU - Kohajda, Zsófia

AU - Horváth, András

AU - Seprényi, György

AU - Kovács, Mária

AU - Virág, L.

AU - Jost, N.

AU - Prorok, János

AU - Ördög, Balázs

AU - Doleschall, Zoltán

AU - Nattel, Stanley

AU - Varró, A.

AU - Bösze, Z.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Background and purpose The reliable assessment of proarrhythmic risk of compounds under development remains an elusive goal. Current safety guidelines focus on the effects of blocking the KCNH2/HERG ion channel-in tissues and animals with intact repolarization. Novel models with better predictive value are needed that more closely reflect the conditions in patients with cardiac remodelling and reduced repolarization reserve. Experimental Approach We have developed a model for the long QT syndrome type-5 in rabbits (LQT5) with cardiac-specific overexpression of a mutant (G52R) KCNE1 β-subunit of the channel that carries the slow delayed-rectifier K+-current (IKs). ECG parameters, including short-term variability of the QT interval (STVQT), a biomarker for proarrhythmic risk, and arrhythmia development were recorded. In vivo, arrhythmia susceptibility was evaluated by i.v. administration of the IKr blocker dofetilide. K+ currents were measured with the patch-clamp technique. Key Results Patch-clamp studies in ventricular myocytes isolated from LQT5 rabbits revealed accelerated IKs and IKr deactivation kinetics. At baseline, LQT5 animals exhibited slightly but significantly prolonged heart-rate corrected QT index (QTi) and increased STVQT. Dofetilide provoked Torsade-de-Pointes arrhythmia in a greater proportion of LQT5 rabbits, paralleled by a further increase in STVQT. Conclusion and Implications We have created a novel transgenic LQT5 rabbit model with increased susceptibility to drug-induced arrhythmias that may represent a useful model for testing proarrhythmic potential and for investigations of the mechanisms underlying arrhythmias and sudden cardiac death due to repolarization disturbances.

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