A novel normalized retention factor in micellar electrokinetic chromatography

M. Idei, Erika Gyorffy, E. Kiss, L. Őrfi, J. Seprődi, Boróka Tamás, F. Hollósy, György Mészáros, G. Kéri

Research output: Article

6 Citations (Scopus)

Abstract

Characteristic properties of the expression k'' = (t(m)-t(o))/(t(mc)- t(o)) and its applicability in micellar electrokinetic capillary chromatography (MEKC) were compared to the previous expression, k' = (t(m)- t(o))/t(o)(1-t(m)/t(mc)), introduced by Terabe. It was proved with theoretical calculation (curve shape analysis) that the properties of function k'' are in full accordance with the properties of the MEKC system and k'' could be applied advantageously to characterize hydrophobicity of the analytes. This conclusion is now supported by experimental data obtained with homolog series of alkylbenzenes and alkylphenones as well as with hydrophobic protected peptides. Migration times, k', k'' values, and software-calculated hydrophobicity data are summarized and analyzed in the present study. Since k'' is a normalized parameter, good relationships between the migration time, the software-calculated hydrophobicity, and the k'' values were obtained. Differences in hydrophobicity of the analytes could be estimated in a more realistic way with the aid of function k'' than by using function k'. Hydrophobicity data estimated on the basis of the k'' values proved to be in good accordance with the expectations based on the migration times and on the chemical structures of the compounds investigated.

Original languageEnglish
Pages (from-to)1561-1567
Number of pages7
JournalElectrophoresis
Volume20
Issue number7
DOIs
Publication statusPublished - 1999

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Hydrophobicity
Chromatography
Hydrophobic and Hydrophilic Interactions
Micellar Electrokinetic Capillary Chromatography
Software
Peptides

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

A novel normalized retention factor in micellar electrokinetic chromatography. / Idei, M.; Gyorffy, Erika; Kiss, E.; Őrfi, L.; Seprődi, J.; Tamás, Boróka; Hollósy, F.; Mészáros, György; Kéri, G.

In: Electrophoresis, Vol. 20, No. 7, 1999, p. 1561-1567.

Research output: Article

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AU - Gyorffy, Erika

AU - Kiss, E.

AU - Őrfi, L.

AU - Seprődi, J.

AU - Tamás, Boróka

AU - Hollósy, F.

AU - Mészáros, György

AU - Kéri, G.

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