A new ochratoxin a biodegradation strategy using cupriavidus basilensis Or16 strain

Szilamér Ferenczi, Mátyás Cserháti, Csilla Krifaton, Sándor Szoboszlay, József Kukolya, Zsuzsanna Szoke, Balázs Koszegi, Mihály Albert, Teréz Barna, M. Mézes, K. Kovács, Balázs Kriszt

Research output: Article

11 Citations (Scopus)

Abstract

Ochratoxin-A (OTA) is a mycotoxin with possibly carcinogenic and nephrotoxic effects in humans and animals. OTA is often found as a contaminant in agricultural commodities. The aim of the present work was to evaluate OTA-degrading and detoxifying potential of Cupriavidus basilensis OR16 strain. In vivo administration of OTA in CD1 male mice (1 or 10 mg/kg body weight for 72 hours or 0.5 mg/kg body weight for 21 days) resulted in significant elevation of OTA levels in the blood, histopathological alterations- and transcriptional changes in OTA-dependent genes (annexinA2, clusterin, sulphotransferase and gadd45 and gadd153) in the renal cortex. These OTA-induced changes were not seen in animals that have been treated with culture supernatants in which OTA was incubated with Cupriavidus basilensis OR16 strain for 5 days. HPLC and ELISA methods identified ochratoxin a as the major metabolite of OTA in Cupriavidus basilensisOR16 cultures, which is not toxic in vivo. This study has demonstrated that Cupriavidus basilensis OR16 efficiently degrade OTA without producing toxic adventitious metabolites.

Original languageEnglish
Article numbere109817
JournalPLoS One
Volume9
Issue number10
DOIs
Publication statusPublished - okt. 10 2014

Fingerprint

Cupriavidus basilensis
Cupriavidus
Ochratoxins
ochratoxins
ochratoxin A
Biodegradation
biodegradation
Poisons
Metabolites
Animals
Body Weight
Clusterin
metabolites
sulfotransferases
Sulfotransferases
body weight
Mycotoxins
agricultural products

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Ferenczi, S., Cserháti, M., Krifaton, C., Szoboszlay, S., Kukolya, J., Szoke, Z., ... Kriszt, B. (2014). A new ochratoxin a biodegradation strategy using cupriavidus basilensis Or16 strain. PLoS One, 9(10), [e109817]. https://doi.org/10.1371/journal.pone.0109817

A new ochratoxin a biodegradation strategy using cupriavidus basilensis Or16 strain. / Ferenczi, Szilamér; Cserháti, Mátyás; Krifaton, Csilla; Szoboszlay, Sándor; Kukolya, József; Szoke, Zsuzsanna; Koszegi, Balázs; Albert, Mihály; Barna, Teréz; Mézes, M.; Kovács, K.; Kriszt, Balázs.

In: PLoS One, Vol. 9, No. 10, e109817, 10.10.2014.

Research output: Article

Ferenczi, S, Cserháti, M, Krifaton, C, Szoboszlay, S, Kukolya, J, Szoke, Z, Koszegi, B, Albert, M, Barna, T, Mézes, M, Kovács, K & Kriszt, B 2014, 'A new ochratoxin a biodegradation strategy using cupriavidus basilensis Or16 strain', PLoS One, vol. 9, no. 10, e109817. https://doi.org/10.1371/journal.pone.0109817
Ferenczi S, Cserháti M, Krifaton C, Szoboszlay S, Kukolya J, Szoke Z et al. A new ochratoxin a biodegradation strategy using cupriavidus basilensis Or16 strain. PLoS One. 2014 okt. 10;9(10). e109817. https://doi.org/10.1371/journal.pone.0109817
Ferenczi, Szilamér ; Cserháti, Mátyás ; Krifaton, Csilla ; Szoboszlay, Sándor ; Kukolya, József ; Szoke, Zsuzsanna ; Koszegi, Balázs ; Albert, Mihály ; Barna, Teréz ; Mézes, M. ; Kovács, K. ; Kriszt, Balázs. / A new ochratoxin a biodegradation strategy using cupriavidus basilensis Or16 strain. In: PLoS One. 2014 ; Vol. 9, No. 10.
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