A multidirectional investigation of stem bark extracts of four African plants: HPLC-MS/MS profiling and biological potentials

Kouadio Bene, Kouadio Ibrahime Sinan, Gokhan Zengin, Alina Diuzheva, J. Jekó́, Zoltán Cziáky, Muhammad Zakariyyah Aumeeruddy, Jianbo Xiao, Mohamad Fawzi Mahomoodally

Research output: Article

1 Citation (Scopus)

Abstract

The practice of traditional medicine, especially herbal medicine, is still prevalent across the African continent. Yet, their in-depth pharmacological and chemical exploitation by the scientific community remain a necessity. The aim of the present study was to investigate into the phenolic components, antioxidant, and enzyme-inhibitory activities of three solvent extracts (ethyl acetate, methanol, and water) of the stem bark of four African plant species (Senna siamea, Distemonanthus benthamianus, Harrisonia abyssinica, and Pycnanthus angolensis). It was found that D. benthamianus followed by P. angolensis, displayed the highest DPPH and ABTS scavenging, ferric and cupric reducing, and total antioxidant capacity in the phosphomolybdenum assay. A similar result was observed for AChE, BChE, and tyrosinase inhibition. The two plants also showed comparable α-amylase inhibitory effect. On the other hand, H. abyssinica showed high metal chelating and α-glucosidase inhibition. Among the solvents used, the methanol extract seemed to be the most bioactive. In addition, TPC was highest in D. benthamianus (135.33–192.29 mg GAE/g) while P. angolensis was richest in TFC (7.68–12.48 mg RE/g). A range of bioactive compounds were identified in the extracts, with variations observed among the plants. Senna siamea stem bark showed the presence of nine compounds; where flavonoids (e.g. naringenin, kaempferol, dihydroquercetin) were recorded. Genistein (m/z 271.06), procyanidin B (m/z 577.13) and C (m/z 865.19) isomers were common in stem barks extracts of D. benthamianus and P. Angolensis. To conclude, D. benthamianus and P. angolensis can be considered as potential pharmaceutical agents or functional food components that could reduce the risks of oxidative stress-related disorders.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume168
DOIs
Publication statusPublished - máj. 10 2019

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Bioelectric potentials
Methanol
Simaroubaceae
Senna Plant
Antioxidants
High Pressure Liquid Chromatography
Glucosidases
Functional Food
Oxidative stress
Monophenol Monooxygenase
Herbal Medicine
Genistein
Scavenging
Enzyme activity
Traditional Medicine
Amylases
Chelation
Flavonoids
Isomers
Medicine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

A multidirectional investigation of stem bark extracts of four African plants : HPLC-MS/MS profiling and biological potentials. / Bene, Kouadio; Sinan, Kouadio Ibrahime; Zengin, Gokhan; Diuzheva, Alina; Jekó́, J.; Cziáky, Zoltán; Aumeeruddy, Muhammad Zakariyyah; Xiao, Jianbo; Mahomoodally, Mohamad Fawzi.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 168, 10.05.2019, p. 217-224.

Research output: Article

Bene, Kouadio ; Sinan, Kouadio Ibrahime ; Zengin, Gokhan ; Diuzheva, Alina ; Jekó́, J. ; Cziáky, Zoltán ; Aumeeruddy, Muhammad Zakariyyah ; Xiao, Jianbo ; Mahomoodally, Mohamad Fawzi. / A multidirectional investigation of stem bark extracts of four African plants : HPLC-MS/MS profiling and biological potentials. In: Journal of Pharmaceutical and Biomedical Analysis. 2019 ; Vol. 168. pp. 217-224.
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AU - Diuzheva, Alina

AU - Jekó́, J.

AU - Cziáky, Zoltán

AU - Aumeeruddy, Muhammad Zakariyyah

AU - Xiao, Jianbo

AU - Mahomoodally, Mohamad Fawzi

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AB - The practice of traditional medicine, especially herbal medicine, is still prevalent across the African continent. Yet, their in-depth pharmacological and chemical exploitation by the scientific community remain a necessity. The aim of the present study was to investigate into the phenolic components, antioxidant, and enzyme-inhibitory activities of three solvent extracts (ethyl acetate, methanol, and water) of the stem bark of four African plant species (Senna siamea, Distemonanthus benthamianus, Harrisonia abyssinica, and Pycnanthus angolensis). It was found that D. benthamianus followed by P. angolensis, displayed the highest DPPH and ABTS scavenging, ferric and cupric reducing, and total antioxidant capacity in the phosphomolybdenum assay. A similar result was observed for AChE, BChE, and tyrosinase inhibition. The two plants also showed comparable α-amylase inhibitory effect. On the other hand, H. abyssinica showed high metal chelating and α-glucosidase inhibition. Among the solvents used, the methanol extract seemed to be the most bioactive. In addition, TPC was highest in D. benthamianus (135.33–192.29 mg GAE/g) while P. angolensis was richest in TFC (7.68–12.48 mg RE/g). A range of bioactive compounds were identified in the extracts, with variations observed among the plants. Senna siamea stem bark showed the presence of nine compounds; where flavonoids (e.g. naringenin, kaempferol, dihydroquercetin) were recorded. Genistein (m/z 271.06), procyanidin B (m/z 577.13) and C (m/z 865.19) isomers were common in stem barks extracts of D. benthamianus and P. Angolensis. To conclude, D. benthamianus and P. angolensis can be considered as potential pharmaceutical agents or functional food components that could reduce the risks of oxidative stress-related disorders.

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