A LC-MS/MS confirmatory method for determination of chloramphenicol in real samples screened by competitive immunoassay

A. Tölgyesi, J. Fekete, V. K. Sharma, E. Pálffi, K. Békési, D. Lukonics, G. Pleva

Research output: Article

1 Citation (Scopus)

Abstract

A new liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to confirm chloramphenicol (CAP) residues in foods of animal origin and in urine samples, which were earlier found positive under the screening analysis, performed by competitive enzyme-linked immunoassay (ELISA) technique. The developed LC-MS/MS method was applied to four non-compliant samples from 2008 to 2012; giving concentrations of CAP residues from 1.18 to 3.68 μg kg-1. All samples, qualified positive by ELISA, were confirmed with the LC-MS/MS technique and found to be non-compliant. The effectiveness of the confirmatory method was proven by participating in a successful proficiency test in year 2010. Both LC-MS/MS and ELISA methods were validated according to the European Union 2002/657/EC decision. The decision limit of the confirmatory method was determined as 0.02 μg kg-1 for CAP in each validated matrix, while the detection capability of the screening test was 0.15 μg kg-1.

Original languageEnglish
Pages (from-to)306-314
Number of pages9
JournalActa Alimentaria
Volume43
Issue number2
DOIs
Publication statusPublished - jún. 1 2014

ASJC Scopus subject areas

  • Food Science

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