A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO

Sue A. Roberts, Günter F. Wildner, Gregor Grass, Andrzej Weichsel, A. Ambrus, Christopher Rensing, William R. Montfort

Research output: Article

112 Citations (Scopus)

Abstract

CueO, a multicopper oxidase, is part of the copper-regulatory cue operon in Escherichia coli, is expressed under conditions of copper stress and shows enhanced oxidase activity when additional copper is present. The 1.7-Å resolution structure of a crystal soaked in CuCl2 reveals a Cu(II) ion bound to the protein 7.5 Å from the T1 copper site in a region rich in methionine residues. The trigonal bipyramidal coordination sphere is unusual, containing two methionine sulfur atoms, two aspartate carboxylate oxygen atoms, and a water molecule. Asp-439 both ligates the labile copper and hydrogen-bonds to His-443, which ligates the T1 copper. This arrangement may mediate electron transfer from substrates to the T1 copper. Mutation of residues bound to the labile copper results in loss of oxidase activity and of copper tolerance, confirming a regulatory role for this site. The methionine-rich portion of the protein, which is similar to that of other proteins involved in copper homeostasis, does not display additional copper binding. The type 3 copper atoms of the trinuclear cluster in the structure are bridged by a chloride ion that completes a square planar coordination sphere for the T2 copper atom but does not affect oxidase activity.

Original languageEnglish
Pages (from-to)31958-31963
Number of pages6
JournalJournal of Biological Chemistry
Volume278
Issue number34
DOIs
Publication statusPublished - aug. 22 2003

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Copper
Oxidoreductases
Ions
Methionine
Atoms
Proteins
Operon
Sulfur
Aspartic Acid
Cues
Chlorides
Escherichia coli
Hydrogen
Homeostasis
Hydrogen bonds
Electrons
Oxygen
Mutation
Water
Crystals

ASJC Scopus subject areas

  • Biochemistry

Cite this

Roberts, S. A., Wildner, G. F., Grass, G., Weichsel, A., Ambrus, A., Rensing, C., & Montfort, W. R. (2003). A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO. Journal of Biological Chemistry, 278(34), 31958-31963. https://doi.org/10.1074/jbc.M302963200

A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO. / Roberts, Sue A.; Wildner, Günter F.; Grass, Gregor; Weichsel, Andrzej; Ambrus, A.; Rensing, Christopher; Montfort, William R.

In: Journal of Biological Chemistry, Vol. 278, No. 34, 22.08.2003, p. 31958-31963.

Research output: Article

Roberts, SA, Wildner, GF, Grass, G, Weichsel, A, Ambrus, A, Rensing, C & Montfort, WR 2003, 'A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO', Journal of Biological Chemistry, vol. 278, no. 34, pp. 31958-31963. https://doi.org/10.1074/jbc.M302963200
Roberts, Sue A. ; Wildner, Günter F. ; Grass, Gregor ; Weichsel, Andrzej ; Ambrus, A. ; Rensing, Christopher ; Montfort, William R. / A labile regulatory copper ion lies near the T1 copper site in the multicopper oxidase CueO. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 34. pp. 31958-31963.
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