A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide

Eda Altan, Steven V. Kubiski, Ákos Boros, Gábor Reuter, Mohammadreza Sadeghi, Xutao Deng, Erica K. Creighton, Marcus J. Crim, Eric Delwart

Research output: Article

5 Citations (Scopus)

Abstract

Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.

Original languageEnglish
Pages (from-to)291-299
Number of pages9
JournalZebrafish
Volume16
Issue number3
DOIs
Publication statusPublished - jún. 2019

    Fingerprint

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Cite this

Altan, E., Kubiski, S. V., Boros, Á., Reuter, G., Sadeghi, M., Deng, X., Creighton, E. K., Crim, M. J., & Delwart, E. (2019). A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide. Zebrafish, 16(3), 291-299. https://doi.org/10.1089/zeb.2018.1710