A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide

Eda Altan, Steven V. Kubiski, Ákos Boros, G. Reuter, Mohammadreza Sadeghi, Xutao Deng, Erica K. Creighton, Marcus J. Crim, Eric Delwart

Research output: Article

1 Citation (Scopus)

Abstract

Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.

Original languageEnglish
Pages (from-to)291-299
Number of pages9
JournalZebrafish
Volume16
Issue number3
DOIs
Publication statusPublished - jún. 1 2019

Fingerprint

Picornaviridae
research institutions
Zebrafish
Danio rerio
epithelium
Epithelium
digestive system
Research
Picornaviridae Infections
Vertebrates
Northern Asia
vertebrates
infection
Biological Phenomena
Host-Pathogen Interactions
Metagenomics
Asymptomatic Infections
capsid
host-pathogen relationships
Enterocytes

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

Cite this

A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide. / Altan, Eda; Kubiski, Steven V.; Boros, Ákos; Reuter, G.; Sadeghi, Mohammadreza; Deng, Xutao; Creighton, Erica K.; Crim, Marcus J.; Delwart, Eric.

In: Zebrafish, Vol. 16, No. 3, 01.06.2019, p. 291-299.

Research output: Article

Altan, E, Kubiski, SV, Boros, Á, Reuter, G, Sadeghi, M, Deng, X, Creighton, EK, Crim, MJ & Delwart, E 2019, 'A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide', Zebrafish, vol. 16, no. 3, pp. 291-299. https://doi.org/10.1089/zeb.2018.1710
Altan, Eda ; Kubiski, Steven V. ; Boros, Ákos ; Reuter, G. ; Sadeghi, Mohammadreza ; Deng, Xutao ; Creighton, Erica K. ; Crim, Marcus J. ; Delwart, Eric. / A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide. In: Zebrafish. 2019 ; Vol. 16, No. 3. pp. 291-299.
@article{dd2c14d3ea0c492c82961fe8084699aa,
title = "A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide",
abstract = "Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20{\%} in their P1 capsids and <36{\%} in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56{\%}) institutions tested. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.",
keywords = "Cyprivirus, Danio rerio, metagenomics, Picornaviridae, picornavirus, zebrafish",
author = "Eda Altan and Kubiski, {Steven V.} and {\'A}kos Boros and G. Reuter and Mohammadreza Sadeghi and Xutao Deng and Creighton, {Erica K.} and Crim, {Marcus J.} and Eric Delwart",
year = "2019",
month = "6",
day = "1",
doi = "10.1089/zeb.2018.1710",
language = "English",
volume = "16",
pages = "291--299",
journal = "Zebrafish",
issn = "1545-8547",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - A Highly Divergent Picornavirus Infecting the Gut Epithelia of Zebrafish (Danio rerio) in Research Institutions Worldwide

AU - Altan, Eda

AU - Kubiski, Steven V.

AU - Boros, Ákos

AU - Reuter, G.

AU - Sadeghi, Mohammadreza

AU - Deng, Xutao

AU - Creighton, Erica K.

AU - Crim, Marcus J.

AU - Delwart, Eric

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.

AB - Zebrafish have been extensively used as a model system for research in vertebrate development and pathogen-host interactions. We describe the complete genome of a novel picornavirus identified during a viral metagenomics analysis of zebrafish gut tissue. The closest relatives of this virus showed identity of <20% in their P1 capsids and <36% in their RdRp qualifying zebrafish picornavirus-1 (ZfPV-1) as member of a novel genus with a proposed name of Cyprivirus. Reverse transcription (RT)-PCR testing of zebrafish from North America, Europe, and Asia showed ZfPV-1 to be globally distributed, being detected in 23 of 41 (56%) institutions tested. In situ hybridization of whole zebrafish showed viral RNA was restricted to a subset of enterocytes and cells in the subjacent lamina propria of the intestine and the intestinal mucosa. This naturally occurring and apparently asymptomatic infection (in wild-type zebrafish lineage AB) provides a natural infection system to study picornavirus-host interactions in an advanced vertebrate model organism. Whether ZfPV-1 infection affects any immunological, developmental, or other biological processes in wild-type or mutant zebrafish lineages remains to be determined.

KW - Cyprivirus

KW - Danio rerio

KW - metagenomics

KW - Picornaviridae

KW - picornavirus

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85065546969&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065546969&partnerID=8YFLogxK

U2 - 10.1089/zeb.2018.1710

DO - 10.1089/zeb.2018.1710

M3 - Article

VL - 16

SP - 291

EP - 299

JO - Zebrafish

JF - Zebrafish

SN - 1545-8547

IS - 3

ER -