A hisztokémia egy megdôlni látszó dogmája: Az urátkristályok szövettani kimutathatósága

M. Bély, Miklós Krutsay

Research output: Article

2 Citations (Scopus)

Abstract

In medical practice there are a number of "truths etched in stone" that are passed on from textbook to textbook and learned by generations before they become obsolete. This short study aims to eliminate a misbelief from the diagnosis of gout that is related to the histological detectability of urate deposits. According to the generally accepted thesis, urate crystals obtained from patients with gout are dissolved in formalin solution, therefore, tissue samples should be fixated in alcohol. The authors have found that urate crystals can be detected on conventionally mounted, native (unstained) sections, despite formalin fixation, whereas the great majority of urate crystals are dissolved during haematoxylin-eosin staining. Therefore, for the detection of urate crystals the tissue samples should be examined on native, unstained sections.

Original languageHungarian
Pages (from-to)37-39
Number of pages3
JournalLege Artis Medicinae
Volume23
Issue number1
Publication statusPublished - 2013

Fingerprint

Uric Acid
Textbooks
Gout
Formaldehyde
Hematoxylin
Eosine Yellowish-(YS)
Alcohols
Staining and Labeling

Keywords

  • Fixation
  • Gout
  • Urate crystals

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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title = "A hisztok{\'e}mia egy megd{\^o}lni l{\'a}tsz{\'o} dogm{\'a}ja: Az ur{\'a}tkrist{\'a}lyok sz{\"o}vettani kimutathat{\'o}s{\'a}ga",
abstract = "In medical practice there are a number of {"}truths etched in stone{"} that are passed on from textbook to textbook and learned by generations before they become obsolete. This short study aims to eliminate a misbelief from the diagnosis of gout that is related to the histological detectability of urate deposits. According to the generally accepted thesis, urate crystals obtained from patients with gout are dissolved in formalin solution, therefore, tissue samples should be fixated in alcohol. The authors have found that urate crystals can be detected on conventionally mounted, native (unstained) sections, despite formalin fixation, whereas the great majority of urate crystals are dissolved during haematoxylin-eosin staining. Therefore, for the detection of urate crystals the tissue samples should be examined on native, unstained sections.",
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AU - Krutsay, Miklós

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AB - In medical practice there are a number of "truths etched in stone" that are passed on from textbook to textbook and learned by generations before they become obsolete. This short study aims to eliminate a misbelief from the diagnosis of gout that is related to the histological detectability of urate deposits. According to the generally accepted thesis, urate crystals obtained from patients with gout are dissolved in formalin solution, therefore, tissue samples should be fixated in alcohol. The authors have found that urate crystals can be detected on conventionally mounted, native (unstained) sections, despite formalin fixation, whereas the great majority of urate crystals are dissolved during haematoxylin-eosin staining. Therefore, for the detection of urate crystals the tissue samples should be examined on native, unstained sections.

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