A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC–MS/MS profiles, antioxidant and enzyme inhibitory properties

Gokhan Zengin, Muhammad Zakariyyah Aumeeruddy, Alina Diuzheva, J. Jekó́, Zoltán Cziáky, Aysegul Yıldıztugay, Evren Yıldıztugay, Mohamad Fawzi Mahomoodally

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Abstract

In the quest for new drugs of herbal origin, the ethyl acetate (EAE), methanol (ME), and water (WE) extracts of Crocus chrysanthus (Herb.) Herb. flowers were analyzed for their polyphenolic composition, antioxidant, and enzyme inhibitory potential. WE showed the highest antioxidant activities in all assays including metal chelating, phosphomolybdenum, FRAP, CUPRAC, ABTS and DPPH. EAE was the most effective enzyme inhibitor, exhibiting the highest inhibition against some enzymes linked to Alzheimer's disease (cholinesterases), diabetes mellitus (α-glucosidase and α-amylase) and hyperpigmentation problems (tyrosinase). The highest total phenolics (34.99 mg GAE/g) and flavonoids content (77.58 mg RE/g) were observed in WE and ME, respectively. Eight compounds were identified in EAE, 24 in ME, and 15 in WE. Kaempferol 3-O glucoside was found in all extracts. In conclusion, C. chrysanthus flowers can be suggested as a source of bioactive components with potential use against chronic disorders caused by oxidative stress. Future in-depth studies are recommended to determine the biological effects of isolated compounds from C. chrysanthus to identify the main compounds modulating the observed activities.

???language???English
???pages???581-589
???numberOfPages???9
???journalAssociation???Journal of Pharmaceutical and Biomedical Analysis
???volume???164
???DOIs???
???publicationStatus???Published - febr. 5 2019

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Oxidative stress
Enzyme Inhibitors
Oxidative Stress
Antioxidants
High Pressure Liquid Chromatography
Methanol
Water
Enzymes
Hyperpigmentation
alpha-Glucosidases
Monophenol Monooxygenase
alpha-Amylases
Cholinesterases
Medical problems
Chelation
Flavonoids
Assays
Diabetes Mellitus
Alzheimer Disease
Metals

Keywords

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Pharmaceutical Science
    • Drug Discovery
    • Spectroscopy
    • Clinical Biochemistry

    Cite this

    A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC–MS/MS profiles, antioxidant and enzyme inhibitory properties. / Zengin, Gokhan; Aumeeruddy, Muhammad Zakariyyah; Diuzheva, Alina; Jekó́, J.; Cziáky, Zoltán; Yıldıztugay, Aysegul; Yıldıztugay, Evren; Mahomoodally, Mohamad Fawzi.

    ???in???: Journal of Pharmaceutical and Biomedical Analysis, ???journal.volume??? 164, 05.02.2019, ???pages??? 581-589.

    ???family-name???: Article

    Zengin, Gokhan ; Aumeeruddy, Muhammad Zakariyyah ; Diuzheva, Alina ; Jekó́, J. ; Cziáky, Zoltán ; Yıldıztugay, Aysegul ; Yıldıztugay, Evren ; Mahomoodally, Mohamad Fawzi. / A comprehensive appraisal on Crocus chrysanthus (Herb.) Herb. flower extracts with HPLC–MS/MS profiles, antioxidant and enzyme inhibitory properties. ???in???: Journal of Pharmaceutical and Biomedical Analysis. 2019 ; ???journal.volume??? 164. ???pages??? 581-589.
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    AU - Diuzheva, Alina

    AU - Jekó́, J.

    AU - Cziáky, Zoltán

    AU - Yıldıztugay, Aysegul

    AU - Yıldıztugay, Evren

    AU - Mahomoodally, Mohamad Fawzi

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