A canarypox vector-expressing cytomegalovirus (CMV) phosphoprotein 65 induces long-lasting cytotoxic T cell responses in human CMV-seronegative subjects

Klara Berencsi, Zsofia Gyulai, E. Gönczöl, Steve Pincus, William I. Cox, Susan Michelson, Laszlo Kari, Claude Meric, Michel Cadoz, John Zahradnik, Stuart Starr, Stanley Plotkin

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115 Citations (Scopus)

Abstract

The major matrix phosphoprotein 65 (pp65) of cytomegalovirus (CMV) is an important target of HLA-restricted cytotoxic T cells (CTL) after natural infection. A canarypox-CMV pp65 recombinant was studied for its ability to induce CMV pp65-specific CTL, helper T lymphocytes, and antibodies in a phase I clinical trial. Twenty-one CMV-seronegative adult volunteers were randomized to receive immunizations at months 0, 1, 3, and 6 with either canarypox-CMV pp65 or placebo. In canarypox-CMV pp65-immunized subjects, pp65-specific CTL were elicited after only 2 vaccinations and were present at months 12 and 26 in all subjects tested. Cell-depletion studies indicated that the CTL were phenotype CD8+. Peripheral blood mononuclear cells proliferated in response to stimulation with purified pp65, and antibodies specific for pp65 also were detected. Canarypox-CMV pp65 is the first recombinant vaccine to elicit CMV-specific CTL responses, which suggests the potential usefulness of this approach in preventing disease caused by CMV.

Original languageEnglish
Pages (from-to)1171-1179
Number of pages9
JournalJournal of Infectious Diseases
Volume183
Issue number8
DOIs
Publication statusPublished - ápr. 15 2001

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Berencsi, K., Gyulai, Z., Gönczöl, E., Pincus, S., Cox, W. I., Michelson, S., Kari, L., Meric, C., Cadoz, M., Zahradnik, J., Starr, S., & Plotkin, S. (2001). A canarypox vector-expressing cytomegalovirus (CMV) phosphoprotein 65 induces long-lasting cytotoxic T cell responses in human CMV-seronegative subjects. Journal of Infectious Diseases, 183(8), 1171-1179. https://doi.org/10.1086/319680