• 4589 Citations
  • 24 h-Index
1983 …2018

Research output per year

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Research Output

  • 4589 Citations
  • 24 h-Index
  • 39 Article
  • 3 Review article
  • 2 Chapter
  • 1 Comment/debate
2018

Rational efforts to streamline the escherichia coli genome

Balikó, G., Vernyik, V., Karcagi, I., Györfy, Z., Draskovits, G., Fehér, T. & Pósfai, G., márc. 15 2018, Synthetic Biology: Parts, Devices and Applications. Wiley-VCH Verlag, p. 49-80 32 p.

Research output: Chapter

2017

Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis

Umenhoffer, K., Draskovits, G., Nyerges, Á., Karcagi, I., Bogos, B., Tímár, E., Csörgö, B., Herczeg, R., Nagy, I., Fehér, T., Pál, C. & Pósfai, G., aug. 18 2017, In : ACS Synthetic Biology. 6, 8, p. 1471-1483 13 p.

Research output: Article

19 Citations (Scopus)
2016

A highly precise and portable genome engineering method allows comparison of mutational effects across bacterial species

Nyerges, Á., Csörgo, B., Nagy, I., Bálint, B., Bihari, P., Lázár, V., Apjok, G., Umenhoffer, K., Bogos, B., Pósfai, G. & Pál, C., márc. 1 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 9, p. 2502-2507 6 p.

Research output: Article

58 Citations (Scopus)

Indispensability of Horizontally Transferred Genes and Its Impact on Bacterial Genome Streamlining

Karcagi, I., Draskovits, G., Umenhoffer, K., Fekete, G., Kovács, K., Méhi, O., Balikó, G., Szappanos, B., Györfy, Z., Fehér, T., Bogos, B., Blattner, F. R., Pál, C., Pósfai, G. & Papp, B., máj. 1 2016, In : Molecular biology and evolution. 33, 5, p. 1257-1269 13 p.

Research output: Article

31 Citations (Scopus)

System-level genome editing in microbes

Csörgő, B., Nyerges, Á., Pósfai, G. & Fehér, T., okt. 1 2016, In : Current Opinion in Microbiology. 33, p. 113-122 10 p.

Research output: Review article

11 Citations (Scopus)
2015

Engineered ribosomal RNA operon copy-number variants of E. coli reveal the evolutionary trade-offs shaping rRNA operon number

Gyorfy, Z., Draskovits, G., Vernyik, V., Blattner, F. F., Gaal, T. & Posfai, G., jan. 1 2015, In : Nucleic acids research. 43, 3, p. 1783-1794 12 p.

Research output: Article

35 Citations (Scopus)
2014

Conditional DNA repair mutants enable highly precise genome engineering

Nyerges, Á., Csörgo, B., Nagy, I., Latinovics, D., Szamecz, B., Pósfai, G. & Pál, C., ápr. 2014, In : Nucleic acids research. 42, 8, p. e62

Research output: Article

31 Citations (Scopus)

The dawn of evolutionary genome engineering

Pál, C., Papp, B. & Pósfai, G., júl. 2014, In : Nature Reviews Genetics. 15, 7, p. 504-512 9 p.

Research output: Review article

30 Citations (Scopus)
2013

Bacterial evolution of antibiotic hypersensitivity

Lázár, V., Pal Singh, G., Spohn, R., Nagy, I., Horváth, B., Hrtyan, M., Busa-Fekete, R., Bogos, B., Méhi, O., Csörgo, B., Pósfai, G., Fekete, G., Szappanos, B., Kégl, B., Papp, B. & Pál, C., okt. 29 2013, In : Molecular systems biology. 9, 700.

Research output: Article

134 Citations (Scopus)

DNA vaccines free from mobile DNA

Frisch, D., Novy, R., Landry, C., Kirkpatrick, H., Pósfai, G. & Blattner, F. R., okt. 1 2013, In : Genetic Engineering and Biotechnology News. 33, 17, 1 p.

Research output: Article

1 Citation (Scopus)
2012

Bacteriophage recombineering in the lytic state using the lambda red recombinases

Fehér, T., Karcagi, I., Blattner, F. R. & Pósfai, G., júl. 1 2012, In : Microbial Biotechnology. 5, 4, p. 466-476 11 p.

Research output: Article

22 Citations (Scopus)

Competition between transposable elements and mutator genes in bacteria

Fehér, T., Bogos, B., Méhi, O., Fekete, G., Csörg, B., Kovács, K., Pósfai, G., Papp, B., Hurst, L. D. & Pál, C., okt. 1 2012, In : Molecular biology and evolution. 29, 10, p. 3153-3159 7 p.

Research output: Article

21 Citations (Scopus)

In the fast lane: Large-scale bacterial genome engineering

Fehér, T., Burland, V. & Pósfai, G., júl. 31 2012, In : Journal of Biotechnology. 160, 1-2, p. 72-79 8 p.

Research output: Article

20 Citations (Scopus)

Low-mutation-rate, reduced-genome Escherichia coli: An improved host for faithful maintenance of engineered genetic constructs

Csörgo, B., Fehér, T., Tímár, E., Blattner, F. R. & Pósfai, G., jan. 20 2012, In : Microbial cell factories. 11, 11.

Research output: Article

51 Citations (Scopus)
2010

Reduced evolvability of Escherichia coli MDS42, an IS-less cellular chassis for molecular and synthetic biology applications

Umenhoffer, K., Fehér, T., Balikó, G., Ayaydin, F., Pósfai, J., Blattner, F. R. & Pósfai, G., máj. 21 2010, In : Microbial cell factories. 9, 38.

Research output: Article

61 Citations (Scopus)
2008

The complete genome sequence of Escherichia coli DH10B: Insights into the biology of a laboratory workhorse

Durfee, T., Nelson, R., Baldwin, S., Plunkett, G., Burland, V., Mau, B., Petrosino, J. F., Qin, X., Muzny, D. M., Ayele, M., Gibbs, R. A., Csörgo, B., Pósfai, G., Weinstock, G. M. & Blattner, F. R., ápr. 1 2008, In : Journal of bacteriology. 190, 7, p. 2597-2606 10 p.

Research output: Article

196 Citations (Scopus)
2007

Directed homologous recombination for genome engineering in Escherichia coli

Csörgo, B. & Pósfai, G., nov. 1 2007, In : Acta biologica Hungarica. 58, SUPPL. 1, p. 1-10 10 p.

Research output: Article

Scarless engineering of the Escherichia coli genome

Fehér, T., Karcagi, I., Gyorfy, Z., Umenhoffer, K., Csörgo, B. & Pósfai, G., dec. 1 2007, Microbial Gene Essentiality Protocols and Bioinformatics. p. 251-259 9 p. (Methods in Molecular Biology; vol. 416).

Research output: Chapter

17 Citations (Scopus)

Systematic genome reductions: Theoretical and experimental approaches

Fehér, T., Papp, B., Pál, C. & Pósfai, G., aug. 1 2007, In : Chemical reviews. 107, 8, p. 3498-3513 16 p.

Research output: Review article

35 Citations (Scopus)
2006

Characterization of cycA mutants of Escherichia coli: An assay for measuring in vivo mutation rates

Fehér, T., Cseh, B., Umenhoffer, K., Karcagi, I. & Pósfai, G., márc. 20 2006, In : Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis. 595, 1-2, p. 184-190 7 p.

Research output: Article

26 Citations (Scopus)

Emergent properties of reduced-genome Escherichia coli

Pósfai, G., Plunkett, G., Fehér, T., Frisch, D., Keil, G. M., Umenhoffer, K., Kolisnychenko, V., Stahl, B., Sharma, S. S., De Arruda, M., Burland, V., Harcum, S. W. & Blattner, F. R., máj. 19 2006, In : Science. 312, 5776, p. 1044-1046 3 p.

Research output: Article

449 Citations (Scopus)
2004

Mutagenesis of herpesvirus BACs by allele replacement.

Borst, E. M., Pósfai, G., Pogoda, F. & Messerle, M., 2004, In : Methods in molecular biology (Clifton, N.J.). 256, p. 269-279 11 p.

Research output: Article

14 Citations (Scopus)
2002

Engineering a reduced Escherichia coli genome

Kolisnychenko, V., Plunkett, G., Herring, C. D., Fehér, T., Pósfai, J., Blattner, F. R. & Pósfai, G., ápr. 25 2002, In : Genome Research. 12, 4, p. 640-647 8 p.

Research output: Article

197 Citations (Scopus)

Urease of enterohemorrhagic Escherichia coli: Evidence for regulation by Fur and a trans-acting factor

Heimer, S. R., Welch, R. A., Perna, N. T., Pósfai, G., Evans, P. S., Kaper, J. B., Blattner, F. R. & Mobley, H. L. T., jan. 31 2002, In : Infection and Immunity. 70, 2, p. 1027-1031 5 p.

Research output: Article

29 Citations (Scopus)
2001

Erratum: Genome sequence of enterohaemorrhagic Escherichia coli 0157:H7 (Nature (2001) 409 (529-533))

Perna, N. T., Plunkett, G., Burland, V., Mau, B., Glasner, J. D., Rose, D. J., Mayhew, G. F., Evans, P. S., Gregor, J., Kirkpatrick, H. A., Posfai, G., Hackett, J., Klink, S., Boutin, A., Shao, Y., Miller, L., Grotheck, E. J., Wayne Davis, N., Lim, A., Dimalanta, E. T. & 8 others, Potamousis, K. D., Apodaca, J., Anantharaman, T. S., Lin, J., Yen, G., Schwartz, D. C., Welch, R. A. & Blattner, F. R., márc. 8 2001, In : Nature. 410, 6825, 1 p.

Research output: Comment/debate

Genome sequence of enterohaemorrhagic Escherichia coli O157:H7

Perna, N. T., Plunkett, G., Burland, V., Mau, B., Glasner, J. D., Rose, D. J., Mayhew, G. F., Evans, P. S., Gregor, J., Kirkpatrick, H. A., Pósfai, G., Hackett, J., Klink, S., Boutin, A., Shao, Y., Miller, L., Grotbeck, E. J., Davis, N. W., Lim, A., Dimalanta, E. T. & 8 others, Potamousis, K. D., Apodaca, J., Anantharaman, T. S., Lin, J., Yen, G., Schwartz, D. C., Welch, R. A. & Blattner, F. R., jan. 25 2001, In : Nature. 409, 6819, p. 529-533 5 p.

Research output: Article

1511 Citations (Scopus)

Role of DNA minor groove interactions in substrate recognition by the M.Sinl and M.EcoRII DNA (cytosine-5) methyltransferases

Kiss, A., Pósfai, G., Zsurka, G., Raskó, T. & Venetianer, P., aug. 1 2001, In : Nucleic acids research. 29, 15, p. 3188-3194 7 p.

Research output: Article

11 Citations (Scopus)
1999

Markerless gene replacement in Escherichia coli stimulated by a double-strand break in the chromosome

Pósfai, G., Kolisnychenko, V., Bereczki, Z. & Blattner, F. R., nov. 15 1999, In : Nucleic acids research. 27, 22, p. 4409-4415 7 p.

Research output: Article

181 Citations (Scopus)
1998

Cre/loxP-mediated in vivo excision of large segments from yeast genome and their amplification based on the 2 μm plasmid-derived system

Yoon, Y. G., Pósfai, G., Szybalski, W. & Kim, S. C., nov. 26 1998, In : Gene. 223, 1-2, p. 67-76 10 p.

Research output: Article

10 Citations (Scopus)

Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7

Perna, N. T., Mayhew, G. F., Pósfai, G., Elliott, S., Donnenberg, M. S., Kaper, J. B. & Blattner, F. R., aug. 1998, In : Infection and Immunity. 66, 8, p. 3810-3817 8 p.

Research output: Article

342 Citations (Scopus)
1997
Open Access
170 Citations (Scopus)
1996
24 Citations (Scopus)
1994

In vivo excision and amplification of large segments of the Escherichia coli genome

Pósfai, G., Koob, M., Hradečná, Z., Hasan, N., Filutowicz, M. & Szybalski, W., jún. 25 1994, In : Nucleic acids research. 22, 12, p. 2392-2398 7 p.

Research output: Article

63 Citations (Scopus)
1991

A novel gene-fusing vector: construction of a 5′-GGmCC-specific chimeric methyltransferase, M · BspRI/M · BsuRI

Kim, S. C., Pósfai, G. & Szybalski, W., ápr. 1991, In : Gene. 100, C, p. 45-50 6 p.

Research output: Article

7 Citations (Scopus)

Complementation by detached parts of GGCC-specific DNA methyltransferases

Pósfai, G., Kim, S. C., Szilák, L., Kovács, A. & Venetianer, P., szept. 25 1991, In : Nucleic acids research. 19, 18, p. 4843-4847 5 p.

Research output: Article

15 Citations (Scopus)
1989

Predictive motifs derived from cytosine methyltransferases

Pósfai, J., Bhagwat, A. S., Pósfai, G. & Roberts, R. J., ápr. 11 1989, In : Nucleic acids research. 17, 7, p. 2421-2435 15 p.

Research output: Article

418 Citations (Scopus)
1988

A simple method for locating methylated bases in DNA, as applied to detect asymmetric methylation by M FokIA

Pósfai, G. & Szybalski, W., szept. 15 1988, In : Gene. 69, 1, p. 147-151 5 p.

Research output: Article

22 Citations (Scopus)

A simple method for locating methylated bases in DNA using class-IIS restriction enzymes

Pósfai, G. & Szybalski, W., dec. 25 1988, In : Gene. 74, 1, p. 179-181 3 p.

Research output: Article

17 Citations (Scopus)
21 Citations (Scopus)
1986
6 Citations (Scopus)
1985

Nudeotide sequence of the BsuRI restriction-modification system

Kiss, A., Posfai, G., Keller, C. C., Venetianer, P. & Roberts, R. J., szept. 25 1985, In : Nucleic acids research. 13, 18, p. 6403-6421 19 p.

Research output: Article

98 Citations (Scopus)
1984

High-copy-number derivatives of the plasmid cloning vector pBR322

Boros, I., Pósfai, G. & Venetianer, P., okt. 1984, In : Gene. 30, 1-3, p. 257-260 4 p.

Research output: Article

70 Citations (Scopus)

Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR

Pósfai, G., Baldauf, F., Erdei, S., Pósfai, J., Venetianer, P. & Kiss, A., dec. 11 1984, In : Nucleic acids research. 12, 23, p. 9039-9049 11 p.

Research output: Article

42 Citations (Scopus)
20 Citations (Scopus)
1983

Structure of the Bacillus sphaericus R modification methylase gene

Pósfai, G., Kiss, A., Erdei, S., Pósfai, J. & Venetianer, P., nov. 5 1983, In : Journal of molecular biology. 170, 3, p. 597-610 14 p.

Research output: Article

53 Citations (Scopus)