Zoonotic transmission of reassortant porcine G4P[6] rotaviruses in Hungarian pediatric patients identified sporadically over a 15year period

Hajnalka Papp, Ráka Borzák, Szilvia Farkas, P. Kisfali, György Lengyel, Péter Molnár, B. Melegh, Jelle Matthijnssens, F. Jakab, Vito Martella, K. Bányai

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Genotype G4P[6] Rotavirus A (RVA) strains collected from children admitted to hospital with gastroenteritis over a 15. year period in the pre rotavirus vaccine era in Hungary were characterized in this study. Whole genome sequencing and phylogenetic analysis was performed on eight G4P[6] RVA strains. All these RVA strains shared a fairly conservative genomic configuration (G4-P[6]-I1/I5-R1-C1-M1-A1/A8-N1-T1/T7-E1-H1) and showed striking similarities to porcine and porcine-derived human RVA strains collected worldwide, although genetic relatedness to some common human RVA strains was also seen. The resolution of phylogenetic relationship between porcine and human RVA genes was occasionally low, making the evaluation of host species origin of individual genes sometimes difficult. Yet the whole genome constellations and overall phylogenetic analyses indicated that these eight Hungarian G4P[6] RVA strains may have originated by independent zoonotic transmission, probably from pigs. Future surveillance studies of human and animal RVA should go parallel to enable the distinction between direct interspecies transmission events and those that are coupled with reassortment of cognate genes.

Original languageEnglish
Pages (from-to)71-80
Number of pages10
JournalInfection, Genetics and Evolution
Volume19
DOIs
Publication statusPublished - Oct 2013

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Rotavirus A
Rotavirus
Zoonoses
Swine
Pediatrics
phylogenetics
swine
gene
genome
gastroenteritis
vaccine
relatedness
pig
genomics
genotype
phylogeny
animal
vif Genes
Genome
Rotavirus Vaccines

Keywords

  • Phylogenetic analysis
  • Rotavirus A
  • Surveillance
  • Whole genome sequencing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Zoonotic transmission of reassortant porcine G4P[6] rotaviruses in Hungarian pediatric patients identified sporadically over a 15year period. / Papp, Hajnalka; Borzák, Ráka; Farkas, Szilvia; Kisfali, P.; Lengyel, György; Molnár, Péter; Melegh, B.; Matthijnssens, Jelle; Jakab, F.; Martella, Vito; Bányai, K.

In: Infection, Genetics and Evolution, Vol. 19, 10.2013, p. 71-80.

Research output: Contribution to journalArticle

Papp, Hajnalka ; Borzák, Ráka ; Farkas, Szilvia ; Kisfali, P. ; Lengyel, György ; Molnár, Péter ; Melegh, B. ; Matthijnssens, Jelle ; Jakab, F. ; Martella, Vito ; Bányai, K. / Zoonotic transmission of reassortant porcine G4P[6] rotaviruses in Hungarian pediatric patients identified sporadically over a 15year period. In: Infection, Genetics and Evolution. 2013 ; Vol. 19. pp. 71-80.
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