Zoogloea oleivorans sp. nov., a floc-forming, petroleum hydrocarbon-degrading bacterium isolated from biofilm

Milán Farkas, András Táncsics, Balázs Kriszt, Tibor Benedek, E. Tóth, Zsuzsa Kéki, Péter G. Veres, Sándor Szoboszlay

Research output: Contribution to journalArticle

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Abstract

A floc-forming, Gram-stain-negative, petroleum hydrocarbon-degrading bacterial strain, designated BucT, was isolated from a petroleum hydrocarbon-contaminated site in Hungary. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BucT formed a distinct phyletic lineage within the genus Zoogloea. Its closest relative was found to be Zoogloea caeniEMB43T (97.2 % 16S rRNA gene sequence similarity) followed by Zoogloea oryzae A-7T (95.9 %), Zoogloea ramigera ATCC 19544T (95.5 %) and Zoogloea resiniphila DhA-35T (95.4 %). The level of DNA–DNA relatedness between strain BucT and Z. caeni EMB43T was 31.6 %. Cells of strain BucT are facultatively aerobic, rod-shaped, and motile by means of a polar flagellum. The strain grew at temperatures of 5–35 °C (optimum 25–28 °C), and at pH 6.0–9.0 (optimum 6.5–7.5). The predominant fatty acids were C16 : 0, C10 : 0 3-OH, C12 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH). The major respiratory quinone was ubiquinone-8 (Q-8) and the predominant polar lipid was phosphatidylethanolamine. The genomic DNA G+C content was 63.2 mol%. On the basis of the chemotaxonomic, molecular and phenotypic data, isolate BucT is considered to represent a novel species of the genus Zoogloea, for which the name Zoogloea oleivorans sp. nov. is proposed. The type strain is BucT ( = DSM 28387T = NCAIM B 02570T).

Original languageEnglish
Pages (from-to)274-279
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume65
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

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Zoogloea
Petroleum
petroleum hydrocarbon
Biofilms
Hydrocarbons
petroleum
biofilm
hydrocarbons
Bacteria
bacterium
gene
new species
bacteria
relatedness
genomics
fatty acid
lipid
Zoogloea resiniphila
phylogenetics
DNA

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Medicine(all)

Cite this

Zoogloea oleivorans sp. nov., a floc-forming, petroleum hydrocarbon-degrading bacterium isolated from biofilm. / Farkas, Milán; Táncsics, András; Kriszt, Balázs; Benedek, Tibor; Tóth, E.; Kéki, Zsuzsa; Veres, Péter G.; Szoboszlay, Sándor.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, No. 1, 01.01.2015, p. 274-279.

Research output: Contribution to journalArticle

Farkas, Milán ; Táncsics, András ; Kriszt, Balázs ; Benedek, Tibor ; Tóth, E. ; Kéki, Zsuzsa ; Veres, Péter G. ; Szoboszlay, Sándor. / Zoogloea oleivorans sp. nov., a floc-forming, petroleum hydrocarbon-degrading bacterium isolated from biofilm. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65, No. 1. pp. 274-279.
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