Zonal distribution of transcripts of four hepatic transcription factors in the mature rat liver

K. O. Lindros, T. Oinonen, J. Issakainen, P. Nagy, S. S. Thorgeirsson

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Abstract

Within the liver acinus the majority of genes are expressed in an ascending or descending gradient from the portal to the central vein. The regulatory factors involved in this spatial pattern of gene expression are still poorly understood. Many liver genes are regulated by liver-enriched transcription factors. Here we report on mRNA distribution of four transcription factors in cell lysates obtained from either the periportal or the perivenous region after zone-specific digitonin infusion and by in-situ hybridization. Northern blot analysis revealed that there were slightly more transcripts of C/EBP, HNF1β (n.s.) and HNF4 (p < 0.05), but fewer of HNF3γ (n.s.), in perivenous than in periportal lysates. A somewhat stronger staining in the perivenous region of HNF4 was also seen by in-situ hybridization. The moderate acinar zonation of the mRNAs of these transcription factors suggests that at best they could modulate but not govern the zonated expression of liver genes in the mature liver.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalCell Biology and Toxicology
Volume13
Issue number4-5
DOIs
Publication statusPublished - Sep 16 1997

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Keywords

  • C/EBP
  • HNF4
  • Liver zonation
  • Transcription factors
  • β-actin mRNA

ASJC Scopus subject areas

  • Toxicology
  • Cell Biology
  • Health, Toxicology and Mutagenesis

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