Zonal distribution of transcripts of four hepatic transcription factors in the mature rat liver

K. O. Lindros, T. Oinonen, J. Issakainen, P. Nagy, S. S. Thorgeirsson

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Within the liver acinus the majority of genes are expressed in an ascending or descending gradient from the portal to the central vein. The regulatory factors involved in this spatial pattern of gene expression are still poorly understood. Many liver genes are regulated by liver-enriched transcription factors. Here we report on mRNA distribution of four transcription factors in cell lysates obtained from either the periportal or the perivenous region after zone-specific digitonin infusion and by in-situ hybridization. Northern blot analysis revealed that there were slightly more transcripts of C/EBP, HNF1β (n.s.) and HNF4 (p <0.05), but fewer of HNF3γ (n.s.), in perivenous than in periportal lysates. A somewhat stronger staining in the perivenous region of HNF4 was also seen by in-situ hybridization. The moderate acinar zonation of the mRNAs of these transcription factors suggests that at best they could modulate but not govern the zonated expression of liver genes in the mature liver.

Original languageEnglish
Pages (from-to)257-262
Number of pages6
JournalCell Biology and Toxicology
Volume13
Issue number4-5
DOIs
Publication statusPublished - 1997

Fingerprint

Liver
Rats
Transcription Factors
Genes
In Situ Hybridization
Gene Expression
Digitonin
Messenger RNA
Gene expression
Northern Blotting
Veins
Staining and Labeling

Keywords

  • β-actin mRNA
  • C/EBP
  • HNF4
  • Liver zonation
  • Transcription factors

ASJC Scopus subject areas

  • Cell Biology
  • Toxicology

Cite this

Zonal distribution of transcripts of four hepatic transcription factors in the mature rat liver. / Lindros, K. O.; Oinonen, T.; Issakainen, J.; Nagy, P.; Thorgeirsson, S. S.

In: Cell Biology and Toxicology, Vol. 13, No. 4-5, 1997, p. 257-262.

Research output: Contribution to journalArticle

Lindros, K. O. ; Oinonen, T. ; Issakainen, J. ; Nagy, P. ; Thorgeirsson, S. S. / Zonal distribution of transcripts of four hepatic transcription factors in the mature rat liver. In: Cell Biology and Toxicology. 1997 ; Vol. 13, No. 4-5. pp. 257-262.
@article{26f6c7ee99d2412490d2d59ed9442aad,
title = "Zonal distribution of transcripts of four hepatic transcription factors in the mature rat liver",
abstract = "Within the liver acinus the majority of genes are expressed in an ascending or descending gradient from the portal to the central vein. The regulatory factors involved in this spatial pattern of gene expression are still poorly understood. Many liver genes are regulated by liver-enriched transcription factors. Here we report on mRNA distribution of four transcription factors in cell lysates obtained from either the periportal or the perivenous region after zone-specific digitonin infusion and by in-situ hybridization. Northern blot analysis revealed that there were slightly more transcripts of C/EBP, HNF1β (n.s.) and HNF4 (p <0.05), but fewer of HNF3γ (n.s.), in perivenous than in periportal lysates. A somewhat stronger staining in the perivenous region of HNF4 was also seen by in-situ hybridization. The moderate acinar zonation of the mRNAs of these transcription factors suggests that at best they could modulate but not govern the zonated expression of liver genes in the mature liver.",
keywords = "β-actin mRNA, C/EBP, HNF4, Liver zonation, Transcription factors",
author = "Lindros, {K. O.} and T. Oinonen and J. Issakainen and P. Nagy and Thorgeirsson, {S. S.}",
year = "1997",
doi = "10.1023/A:1007479223229",
language = "English",
volume = "13",
pages = "257--262",
journal = "Cell Biology and Toxicology",
issn = "0742-2091",
publisher = "Springer Netherlands",
number = "4-5",

}

TY - JOUR

T1 - Zonal distribution of transcripts of four hepatic transcription factors in the mature rat liver

AU - Lindros, K. O.

AU - Oinonen, T.

AU - Issakainen, J.

AU - Nagy, P.

AU - Thorgeirsson, S. S.

PY - 1997

Y1 - 1997

N2 - Within the liver acinus the majority of genes are expressed in an ascending or descending gradient from the portal to the central vein. The regulatory factors involved in this spatial pattern of gene expression are still poorly understood. Many liver genes are regulated by liver-enriched transcription factors. Here we report on mRNA distribution of four transcription factors in cell lysates obtained from either the periportal or the perivenous region after zone-specific digitonin infusion and by in-situ hybridization. Northern blot analysis revealed that there were slightly more transcripts of C/EBP, HNF1β (n.s.) and HNF4 (p <0.05), but fewer of HNF3γ (n.s.), in perivenous than in periportal lysates. A somewhat stronger staining in the perivenous region of HNF4 was also seen by in-situ hybridization. The moderate acinar zonation of the mRNAs of these transcription factors suggests that at best they could modulate but not govern the zonated expression of liver genes in the mature liver.

AB - Within the liver acinus the majority of genes are expressed in an ascending or descending gradient from the portal to the central vein. The regulatory factors involved in this spatial pattern of gene expression are still poorly understood. Many liver genes are regulated by liver-enriched transcription factors. Here we report on mRNA distribution of four transcription factors in cell lysates obtained from either the periportal or the perivenous region after zone-specific digitonin infusion and by in-situ hybridization. Northern blot analysis revealed that there were slightly more transcripts of C/EBP, HNF1β (n.s.) and HNF4 (p <0.05), but fewer of HNF3γ (n.s.), in perivenous than in periportal lysates. A somewhat stronger staining in the perivenous region of HNF4 was also seen by in-situ hybridization. The moderate acinar zonation of the mRNAs of these transcription factors suggests that at best they could modulate but not govern the zonated expression of liver genes in the mature liver.

KW - β-actin mRNA

KW - C/EBP

KW - HNF4

KW - Liver zonation

KW - Transcription factors

UR - http://www.scopus.com/inward/record.url?scp=0030808426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030808426&partnerID=8YFLogxK

U2 - 10.1023/A:1007479223229

DO - 10.1023/A:1007479223229

M3 - Article

VL - 13

SP - 257

EP - 262

JO - Cell Biology and Toxicology

JF - Cell Biology and Toxicology

SN - 0742-2091

IS - 4-5

ER -