Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two Brassica species with different sensitivity to zinc stress

Gábor Feigl, Nóra Lehotai, Árpád Molnár, Attila Ördög, Marta Rodríguez-Ruiz, José M. Palma, Francisco J. Corpas, László Erdei, Zsuzsanna Kolbert

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background and Aims Zinc (Zn) is an essential micronutrient naturally present in soils, but anthropogenic activities can lead to accumulation in the environment and resulting damage to plants. Heavy metals such as Zn can induce oxidative stress and the generation of reactive oxygen and nitrogen species (ROS and RNS), which can reduce growth and yield in crop plants. This study assesses the interplay of these two families of molecules in order to evaluate the responses in roots of two Brassica species under high concentrations of Zn. Methods Nine-day-old hydroponically grown Brassica juncea (Indian mustard) and B. napus (oilseed rape) seedlings were treated with ZnSO4 (0, 50, 150 and 300∈μm) for 7∈d. Stress intensity was assessed through analyses of cell wall damage and cell viability. Biochemical and cellular techniques were used to measure key components of the metabolism of ROS and RNS including lipid peroxidation, enzymatic antioxidants, protein nitration and content of superoxide radical (O2·-), nitric oxide (NO) and peroxynitrite (ONOO-). Key Results Analysis of morphological root damage and alterations of microelement homeostasis indicate that B. juncea is more tolerant to Zn stress than B. napus. ROS and RNS parameters suggest that the oxidative components are predominant compared with the nitrosative components in the root system of both species. Conclusions The results indicate a clear relationship between ROS and RNS metabolism as a mechanism of response against stress caused by an excess of Zn. The oxidative stress components seem to be more dominant than the elements of the nitrosative stress in the root system of these two Brassica species.

Original languageEnglish
Pages (from-to)613-625
Number of pages13
JournalAnnals of Botany
Volume116
Issue number4
DOIs
Publication statusPublished - Sep 1 2015

Keywords

  • Brassica juncea
  • Brassica napus
  • Indian mustard
  • RNS
  • ROS
  • excess zinc
  • nitrosative stress
  • oilseed rape
  • oxidative stress
  • protein nitration
  • reactive nitrogen species
  • reactive oxygen species

ASJC Scopus subject areas

  • Plant Science

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