Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two Brassica species with different sensitivity to zinc stress

Gábor Feigl, Nóra Lehotai, Árpád Molnár, Attila Ördög, Marta Rodríguez-Ruiz, José M. Palma, Francisco J. Corpas, L. Erdei, Zsuzsanna Kolbert

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background and Aims Zinc (Zn) is an essential micronutrient naturally present in soils, but anthropogenic activities can lead to accumulation in the environment and resulting damage to plants. Heavy metals such as Zn can induce oxidative stress and the generation of reactive oxygen and nitrogen species (ROS and RNS), which can reduce growth and yield in crop plants. This study assesses the interplay of these two families of molecules in order to evaluate the responses in roots of two Brassica species under high concentrations of Zn. Methods Nine-day-old hydroponically grown Brassica juncea (Indian mustard) and B. napus (oilseed rape) seedlings were treated with ZnSO4 (0, 50, 150 and 300∈μm) for 7∈d. Stress intensity was assessed through analyses of cell wall damage and cell viability. Biochemical and cellular techniques were used to measure key components of the metabolism of ROS and RNS including lipid peroxidation, enzymatic antioxidants, protein nitration and content of superoxide radical (O2·-), nitric oxide (NO) and peroxynitrite (ONOO-). Key Results Analysis of morphological root damage and alterations of microelement homeostasis indicate that B. juncea is more tolerant to Zn stress than B. napus. ROS and RNS parameters suggest that the oxidative components are predominant compared with the nitrosative components in the root system of both species. Conclusions The results indicate a clear relationship between ROS and RNS metabolism as a mechanism of response against stress caused by an excess of Zn. The oxidative stress components seem to be more dominant than the elements of the nitrosative stress in the root system of these two Brassica species.

Original languageEnglish
Pages (from-to)613-625
Number of pages13
JournalAnnals of Botany
Volume116
Issue number4
DOIs
Publication statusPublished - Sep 1 2015

Fingerprint

Brassica
zinc
oxygen
Brassica juncea
metabolism
nitrogen
Brassica napus
root systems
oxidative stress
dietary minerals
superoxide anion
anthropogenic activities
cell viability
nitric oxide
trace elements
stress response
homeostasis
heavy metals
lipid peroxidation
cell walls

Keywords

  • Brassica juncea
  • Brassica napus
  • excess zinc
  • Indian mustard
  • nitrosative stress
  • oilseed rape
  • oxidative stress
  • protein nitration
  • reactive nitrogen species
  • reactive oxygen species
  • RNS
  • ROS

ASJC Scopus subject areas

  • Plant Science

Cite this

Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two Brassica species with different sensitivity to zinc stress. / Feigl, Gábor; Lehotai, Nóra; Molnár, Árpád; Ördög, Attila; Rodríguez-Ruiz, Marta; Palma, José M.; Corpas, Francisco J.; Erdei, L.; Kolbert, Zsuzsanna.

In: Annals of Botany, Vol. 116, No. 4, 01.09.2015, p. 613-625.

Research output: Contribution to journalArticle

Feigl, Gábor ; Lehotai, Nóra ; Molnár, Árpád ; Ördög, Attila ; Rodríguez-Ruiz, Marta ; Palma, José M. ; Corpas, Francisco J. ; Erdei, L. ; Kolbert, Zsuzsanna. / Zinc induces distinct changes in the metabolism of reactive oxygen and nitrogen species (ROS and RNS) in the roots of two Brassica species with different sensitivity to zinc stress. In: Annals of Botany. 2015 ; Vol. 116, No. 4. pp. 613-625.
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abstract = "Background and Aims Zinc (Zn) is an essential micronutrient naturally present in soils, but anthropogenic activities can lead to accumulation in the environment and resulting damage to plants. Heavy metals such as Zn can induce oxidative stress and the generation of reactive oxygen and nitrogen species (ROS and RNS), which can reduce growth and yield in crop plants. This study assesses the interplay of these two families of molecules in order to evaluate the responses in roots of two Brassica species under high concentrations of Zn. Methods Nine-day-old hydroponically grown Brassica juncea (Indian mustard) and B. napus (oilseed rape) seedlings were treated with ZnSO4 (0, 50, 150 and 300∈μm) for 7∈d. Stress intensity was assessed through analyses of cell wall damage and cell viability. Biochemical and cellular techniques were used to measure key components of the metabolism of ROS and RNS including lipid peroxidation, enzymatic antioxidants, protein nitration and content of superoxide radical (O2·-), nitric oxide (NO) and peroxynitrite (ONOO-). Key Results Analysis of morphological root damage and alterations of microelement homeostasis indicate that B. juncea is more tolerant to Zn stress than B. napus. ROS and RNS parameters suggest that the oxidative components are predominant compared with the nitrosative components in the root system of both species. Conclusions The results indicate a clear relationship between ROS and RNS metabolism as a mechanism of response against stress caused by an excess of Zn. The oxidative stress components seem to be more dominant than the elements of the nitrosative stress in the root system of these two Brassica species.",
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AU - Lehotai, Nóra

AU - Molnár, Árpád

AU - Ördög, Attila

AU - Rodríguez-Ruiz, Marta

AU - Palma, José M.

AU - Corpas, Francisco J.

AU - Erdei, L.

AU - Kolbert, Zsuzsanna

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AB - Background and Aims Zinc (Zn) is an essential micronutrient naturally present in soils, but anthropogenic activities can lead to accumulation in the environment and resulting damage to plants. Heavy metals such as Zn can induce oxidative stress and the generation of reactive oxygen and nitrogen species (ROS and RNS), which can reduce growth and yield in crop plants. This study assesses the interplay of these two families of molecules in order to evaluate the responses in roots of two Brassica species under high concentrations of Zn. Methods Nine-day-old hydroponically grown Brassica juncea (Indian mustard) and B. napus (oilseed rape) seedlings were treated with ZnSO4 (0, 50, 150 and 300∈μm) for 7∈d. Stress intensity was assessed through analyses of cell wall damage and cell viability. Biochemical and cellular techniques were used to measure key components of the metabolism of ROS and RNS including lipid peroxidation, enzymatic antioxidants, protein nitration and content of superoxide radical (O2·-), nitric oxide (NO) and peroxynitrite (ONOO-). Key Results Analysis of morphological root damage and alterations of microelement homeostasis indicate that B. juncea is more tolerant to Zn stress than B. napus. ROS and RNS parameters suggest that the oxidative components are predominant compared with the nitrosative components in the root system of both species. Conclusions The results indicate a clear relationship between ROS and RNS metabolism as a mechanism of response against stress caused by an excess of Zn. The oxidative stress components seem to be more dominant than the elements of the nitrosative stress in the root system of these two Brassica species.

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