Zeaxanthin and echinenone modify the structure of photosystem I trimer in Synechocystis sp. PCC 6803

Sindhujaa Vajravel, Mihály Kis, Kinga Kłodawska, Hajnalka Laczko-Dobos, Przemysław Malec, László Kovács, Z. Gombos, Tunde N. Toth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The function of xanthophylls in the organisation and structure of the photosynthetic complexes is not completely clarified yet. Recently, we observed a reduced level of the photosystem oligomers upon xanthophyll deficiency, although xanthophylls are not considered to be part of the photosynthetic complexes of cyanobacteria. The present study aimed at further investigating the relationship between xanthophylls and photosytem I (PSI) complex in the cyanobacterium Synechocystis sp. PCC 6803. Interestingly, we recorded the presence of echinenone and zeaxanthin in the isolated PSI trimers. These two xanthophyll species are among the most abundant xanthophylls in this cyanobacterial species. Various xanthophyll biosynthesis mutants were used to investigate the specific role of these xanthophylls. Our spectroscopic results revealed specific structural changes manifested in altered pigment-pigment or pigment-protein interactions within PSI complex in the absence of zeaxanthin and echinenone. These structural modifications of the complexes seem to destabilize the PSI trimeric complexes and eventually result in an increased propensity for monomerization. Our results clearly demonstrate that xanthophylls are important for the fine-tuning of the PSI trimer structure. These xanthophylls could be part of the complex or be embedded in the membrane in the vicinity of PSI.

Original languageEnglish
Pages (from-to)510-518
Number of pages9
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1858
Issue number7
DOIs
Publication statusPublished - Jul 1 2017

Fingerprint

Xanthophylls
Synechocystis
Photosystem I Protein Complex
Pigments
Photosynthetic Reaction Center Complex Proteins
Cyanobacteria
echinenone
Zeaxanthins
Biosynthesis
Oligomers
Tuning

Keywords

  • Carotenoid
  • Cyanobacteria
  • Echinenone
  • Photosynthesis
  • Photosystem I
  • Zeaxanthin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Vajravel, S., Kis, M., Kłodawska, K., Laczko-Dobos, H., Malec, P., Kovács, L., ... Toth, T. N. (2017). Zeaxanthin and echinenone modify the structure of photosystem I trimer in Synechocystis sp. PCC 6803. Biochimica et Biophysica Acta - Bioenergetics, 1858(7), 510-518. https://doi.org/10.1016/j.bbabio.2017.05.001

Zeaxanthin and echinenone modify the structure of photosystem I trimer in Synechocystis sp. PCC 6803. / Vajravel, Sindhujaa; Kis, Mihály; Kłodawska, Kinga; Laczko-Dobos, Hajnalka; Malec, Przemysław; Kovács, László; Gombos, Z.; Toth, Tunde N.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1858, No. 7, 01.07.2017, p. 510-518.

Research output: Contribution to journalArticle

Vajravel, Sindhujaa ; Kis, Mihály ; Kłodawska, Kinga ; Laczko-Dobos, Hajnalka ; Malec, Przemysław ; Kovács, László ; Gombos, Z. ; Toth, Tunde N. / Zeaxanthin and echinenone modify the structure of photosystem I trimer in Synechocystis sp. PCC 6803. In: Biochimica et Biophysica Acta - Bioenergetics. 2017 ; Vol. 1858, No. 7. pp. 510-518.
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